Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway

被引：98

作者：

Adivarahan, Srivathsan ^{[1
]}

Livingston, Nathan ^{[2
]}

Nicholson, Beth ^{[3
]}

Rahman, Samir ^{[1
]}

Wu, Bin ^{[2
]}

Rissland, Olivia S. ^{[3
,4
]}

Zenklusen, Daniel ^{[1
]}

机构：

[1] Univ Montreal, Dept Biochim & Med Mol, Montreal, PQ H3T 1J4, Canada

[2] Johns Hopkins Sch Med, Ctr Cell Dynam, Solomon Snyder Dept Neurosci, Dept Biophys & Biophys Chem, Baltimore, MD 21224 USA

[3] Hosp Sick Children, Mol Med Program, Toronto, ON M5G 0A4, Canada

[4] Univ Colorado, Dept Biochem & Mol Genet, Sch Med, RNA Biosci Initiat, Aurora, CO 80045 USA

来源：

MOLECULAR CELL | 2018年 / 72卷 / 04期

基金：

加拿大创新基金会; 加拿大健康研究院;

关键词：

EUKARYOTIC TRANSLATION INITIATION; RNA-BINDING PROTEINS; MESSENGER-RNA; STRESS GRANULES; PROFILING REVEALS; LIVING CELLS; LIVE CELLS; MOLECULES; POLYSOMES; ENDS;

D O I：

10.1016/j.molcel.2018.10.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

mRNAs form ribonucleoprotein complexes (mRNPs) by association with proteins that are crucial for mRNA metabolism. While the mRNP proteome has been well characterized, little is known about mRNP organization. Using a single-molecule approach, we show that mRNA conformation changes depending on its cellular localization and translational state. Compared to nuclear mRNPs and lncRNPs, association with ribosomes decompacts individual mRNAs, while pharmacologically dissociating ribosomes or sequestering them into stress granules leads to increased compaction. Moreover, translating mRNAs rarely show co-localized 5 0 and 3 0 ends, indicating either that mRNAs are not translated in a closed-loop configuration, or that mRNA circularization is transient, suggesting that a stable closed-loop conformation is not a universal state for all translating mRNAs.

引用

页码：727 / +

页数：17

共 69 条

[1] Conformation transitions of eukaryotic polyribosomes during multi-round translation [J].

Afonina, Zhanna A. ;

Myasnikov, Alexander G. ;

Shirokov, Vladimir A. ;

Klaholz, Bruno P. ;

Spirin, Alexander S. .

NUCLEIC ACIDS RESEARCH, 2015, 43 (01) :618-628

[2] Probing the closed-loop model of mRNA translation in living cells [J].

Archer, Stuart K. ;

Shirokikh, Nikolay E. ;

Hallwirth, Claus V. ;

Beilharz, Traude H. ;

Preiss, Thomas .

RNA BIOLOGY, 2015, 12 (03) :248-254

[3] Purification of Nuclear Poly(A)-binding Protein Nab2 Reveals Association with the Yeast Transcriptome and a Messenger Ribonucleoprotein Core Structure [J].

Batisse, Julien ;

Batisse, Claire ;

Budd, Aidan ;

Boettcher, Bettina ;

Hurt, Ed .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (50) :34911-34917

[4] Targeting the translation machinery in cancer [J].

Bhat, Mamatha ;

Robichaud, Nathaniel ;

Hulea, Laura ;

Sonenberg, Nahum ;

Pelletier, Jerry ;

Topisirovic, Ivan .

NATURE REVIEWS DRUG DISCOVERY, 2015, 14 (04) :261-278

[5] Sizes of Long RNA Molecules Are Determined by the Branching Patterns of Their Secondary Structures [J].

Borodavka, Alexander ;

Singaram, Surendra W. ;

Stockley, Peter G. ;

Gelbart, William M. ;

Ben-Shaul, Avinoam ;

Tuma, Roman .

BIOPHYSICAL JOURNAL, 2016, 111 (10) :2077-2085

[6] Eukaryotic Stress Granules: The Ins and Outs of Translation [J].

Buchan, J. Ross ;

Parker, Roy .

MOLECULAR CELL, 2009, 36 (06) :932-941

[7] Localization and abundance analysis of human IncRNAs at single-cell and single-molecule resolution [J].

Cabili, Moran N. ;

Dunagin, Margaret C. ;

McClanahan, Patrick D. ;

Biaesch, Andrew ;

Padovan-Merhar, Olivia ;

Regev, Aviv ;

Rinn, John L. ;

Raj, Arjun .

GENOME BIOLOGY, 2015, 16

[8] TAIL-seq: Genome-wide Determination of Poly(A) Tail Length and 3′ End Modifications [J].

Chang, Hyeshik ;

Lim, Jaechul ;

Ha, Minju ;

Kim, V. Narry .

MOLECULAR CELL, 2014, 53 (06) :1044-1052

[9] Ionic strength-dependent persistence lengths of single-stranded RNA and DNA [J].

Chen, Huimin ;

Meisburger, Steve P. ;

Pabit, Suzette A. ;

Sutton, Julie L. ;

Webb, Watt W. ;

Pollack, Lois .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (03) :799-804

[10]

Christensen AK, 1999, ANAT REC, V255, P116, DOI 10.1002/(SICI)1097-0185(19990601)255:2<116::AID-AR2>3.0.CO

← 1 2 3 4 5 6 7 →