Dufulin enhances salt resistance of rice

Dufulin is a newly developed plant antiviral agent, which is widely used in the control of many viral crop dis-eases. Existing research mainly focuses on its antiviral effect, but research in relation to resistance to abiotic stress is unclear. This study was based on the treatment of rice with salt (NaCl), and exogenous application of Dufulin as a stress-resistant agent. The effect of Dufulin on salt stress of rice was revealed. There were 1997 differential genes detected, including 1449 up-regulated and 548 down-regulated. After the application of Dufulin to rice, when salt stress was applied, peroxidase activity was increased and superoxide dismutase activity was reduced; GO and KEGG analyses indicated that the stimulated genes are related to the stress resistance pathway, thus improving the ability of rice to resist salt stress. Quantitative real-time PCR analysis was used to verify the dynamic changes of growth-and stress-resistance-related genes, among which integral membrane protein DUF6 containing protein, OsHKT1;4 (Na+ transporter) and zinc-finger protein were verified to increase by more than three times, and OsIAA1 and OsIAA9 were verified as down-regulated. Measuring the length of root, stem and leaf, and OsIAA1 and OsIAA9 expression showed that Dufulin promoted rice growth. After that, Dufulin could enhance the salt resistance of rice by regulating the expression of integral membrane protein DUF6 containing protein, OsHKT1;4, zinc-finger protein and other related genes under salt stress. The results eluci-dated the mechanism of Dufulin action during salt stress in rice at the transcriptional level.