Single-Molecule FRET Reveals a Cooperative Effect of Two Methyl Group Modifications in the Folding of Human Mitochondrial tRNALys

被引：20
作者：
Kobitski, Andrei Yu [1 ,2 ]
Hengesbach, Martin [3 ]
Seidu-Larry, Salifu [3 ]
Dammertz, Kirsten [4 ]
Chow, Christine S. [5 ]
van Aerschot, Arthur [6 ]
Nienhaus, G. Ulrich [1 ,2 ,7 ]
Helm, Mark [3 ,8 ]
机构：
[1] Karlsruhe Inst Technol, Inst Appl Phys, D-76131 Karlsruhe, Germany
[2] Karlsruhe Inst Technol, Ctr Funct Nanostruct, D-76131 Karlsruhe, Germany
[3] Heidelberg Univ, Inst Pharm & Mol Biotechnol, D-69120 Heidelberg, Germany
[4] Univ Ulm, Inst Biophys, D-89069 Ulm, Germany
[5] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA
[6] Katholieke Univ Leuven, B-3000 Louvain, Belgium
[7] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
[8] Johannes Gutenberg Univ Mainz, Inst Pharm & Biochem, D-55128 Mainz, Germany
来源：
CHEMISTRY & BIOLOGY | 2011年 / 18卷 / 07期
关键词：
PHENYLALANINE TRANSFER-RNA; MODIFIED NUCLEOTIDES; SACCHAROMYCES-CEREVISIAE; METHYLTRANSFERASE; 1-METHYLADENOSINE; IDENTIFICATION; SURVEILLANCE; DEGRADATION; NUCLEOSIDES; COMPLEX;
D O I：
10.1016/j.chembiol.2011.03.016
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using a combination of advanced RNA synthesis techniques and single molecule spectroscopy, the deconvolution of individual contributions of post-transcriptional modifications to the overall folding and stabilization of human mitochondrial tRNA(Lys) is described. An unexpected destabilizing effect of two pseudouridines on the native tRNA folding was evidenced. Furthermore, the presence of m(2)G10 alone does not facilitate the folding of tRNA(Lys), but a stabilization of the biologically functional cloverleaf shape in conjunction with the principal stabilizing component m(1)A9 exceeds the contribution of m(1)A alone. This constitutes an unprecedented cooperative effect of two nucleotide modifications in the context of a naturally occurring RNA, which may be of general importance for tRNA structure and help understanding several recently described decay pathways for hypomodified tRNAs.
引用
收藏
页码：928 / 936
页数：9
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