Role of mannose receptor in oligochitosan-mediated stimulation of macrophage function

被引：89
作者：
Han, YP
Zhao, LH [1 ]
Yu, ZJ
Feng, J
Yu, QQ
机构：
[1] Zhejiang Univ, Coll Med, Dept Biochem & Mol Biol, Hangzhou 310027, Peoples R China
[2] Zhejiang Tradit Chinese Med Hosp, Inst Hematol, Hangzhou, Peoples R China
[3] Zhejiang Univ, Coll Med, Affiliated Hosp 2, Inst Orthopaed, Hangzhou 310027, Peoples R China
来源：
INTERNATIONAL IMMUNOPHARMACOLOGY | 2005年 / 5卷 / 10期
基金：
中国国家自然科学基金;
关键词：
oligochitosan; macrophage; mannose receptor; tumor necrosis factor-alpha;
D O I：
10.1016/j.intimp.2005.04.015
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
In this paper, we aimed to study the role of mannose receptor (MR) in oligochitosan induced RAW264.7 (a murine macrophage cell line) activation. Oligochitosan, which has 3-10 saccharide (N-acetyl-glucosamine or glucosamine) residues, was prepared by enzymatic hydrolysis of chitosan using cellulase. Fluorophore 2-aminoacridone was conjugated to oligochitosan to observe cellular events of oligochitosan-RAW264.7 interaction under Confocal Laser Microscopy. RT-PCR was performed to assess the level of tumor necrosis factor-alpha (TNF-alpha) secretion. Free cytosolic Ca2+ was measured using the fluorescent Ca2+ indicator Fluo3/AM. We found that MR was the major receptor responsible for oligochitosan uptake. MR was also engaged in oligochitosan induced TNF-alpha enhancement and [Ca2+](in) flux and may serve as a signaling receptor in oligochitosan-induced activation of macrophage function. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：1533 / 1542
页数：10
相关论文
共 25 条
[1]   Differential responses of human mononuclear phagocytes to mycobacterial lipoarabinomannans: Role of CD14 and the mannose receptor [J].
Bernardo, J ;
Billingslea, AM ;
Blumenthal, RL ;
Seetoo, KF ;
Simons, ER ;
Fenton, MJ .
INFECTION AND IMMUNITY, 1998, 66 (01) :28-35
[2]   WOUND MANAGEMENT WITH N-CARBOXYBUTYL CHITOSAN [J].
BIAGINI, G ;
BERTANI, A ;
MUZZARELLI, R ;
DAMADEI, A ;
DIBENEDETTO, G ;
BELLIGOLLI, A ;
RICCOTTI, G ;
ZUCCHINI, C ;
RIZZOLI, C .
BIOMATERIALS, 1991, 12 (03) :281-286
[3]   Receptor-mediated stimulatory effect of oligochitosan in macrophages [J].
Feng, J ;
Zhao, LH ;
Yu, QQ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 317 (02) :414-420
[4]   SECRETION OF TNF-ALPHA BY ALVEOLAR MACROPHAGES IN RESPONSE TO CANDIDA-ALBICANS MANNAN [J].
GARNER, RE ;
RUBANOWICE, K ;
SAWYER, RT ;
HUDSON, JA .
JOURNAL OF LEUKOCYTE BIOLOGY, 1994, 55 (02) :161-168
[5]   Pattern recognition receptors: Doubling up for the innate immune response [J].
Gordon, S .
CELL, 2002, 111 (07) :927-930
[6]   Antimicrobial effect of chitooligosaccharides produced by bioreactor [J].
Jeon, YJ ;
Park, PJ ;
Kim, SK .
CARBOHYDRATE POLYMERS, 2001, 44 (01) :71-76
[7]   Characterization of mannose receptor-dependent phagocytosis mediated by Mycobacterium tuberculosis lipoarabinomannan [J].
Kang, BK ;
Schlesinger, LS .
INFECTION AND IMMUNITY, 1998, 66 (06) :2769-2777
[8]   Receptor-independent augmentation of adenovirus-mediated gene transfer with chitosan in vitro [J].
Kawamata, Y ;
Nagayama, Y ;
Nakao, K ;
Mizuguchi, H ;
Hayakawa, T ;
Sato, T ;
Ishii, N .
BIOMATERIALS, 2002, 23 (23) :4573-4579
[9]   CHITOSAN OLIGOMERS FROM FUSARIUM-SOLANI PEA INTERACTIONS, CHITINASE BETA-GLUCANASE DIGESTION OF SPORELINGS AND FROM FUNGAL WALL CHITIN ACTIVELY INHIBIT FUNGAL GROWTH AND ENHANCE DISEASE RESISTANCE [J].
KENDRA, DF ;
CHRISTIAN, D ;
HADWIGER, LA .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1989, 35 (03) :215-230
[10]   Nod factors and chitooligomers elicit an increase in cytosolic calcium in aequorin-expressing soybean cells [J].
Müller, J ;
Staehelin, C ;
Xie, ZP ;
Neuhaus-Url, G ;
Boller, T .
PLANT PHYSIOLOGY, 2000, 124 (02) :733-739
123