Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues

被引：80

作者：

Fang, Haixiao ^{[1
,2
]}

Zhang, Hang ^{[1
,2
]}

Li, Lin ^{[1
,2
]}

Ni, Yun ^{[1
,2
]}

Shi, Riri ^{[1
,2
]}

Li, Zheng ^{[1
,2
]}

Yang, Xuekang ^{[3
]}

Ma, Bo ^{[4
]}

Zhang, Chengwu ^{[1
,2
]}

Wu, Qiong ^{[1
,2
]}

Yu, Changmin ^{[1
,2
]}

Yang, Naidi ^{[1
,2
]}

Yao, Shao Q. ^{[5
]}

Huang, Wei ^{[1
,2
,6
,7
]}

机构：

[1] Nanjing Tech Univ NanjingTech, Key Lab Flexible Elect KLOFE, 30 South Puzhu Rd, Nanjing 211816, Peoples R China

[2] Nanjing Tech Univ NanjingTech, Inst Adv Mat, 30 South Puzhu Rd, Nanjing 211816, Peoples R China

[3] Fourth Mil Med Univ, Dept Burns & Cutaneous Surg, Xijing Hosp, Xian 710032, Peoples R China

[4] Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing 210023, Peoples R China

[5] Natl Univ Singapore, Dept Chem, 3 Sci Dr 3, Singapore 117543, Singapore

[6] Northwestern Polytech Univ, Shaanxi Inst Flexible Elect, 127 West Youyi Rd, Xian 710072, Peoples R China

[7] Northwestern Polytech Univ, Inst Biomed Mat & Engn, 127 West Youyi Rd, Xian 710072, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2020年 / 59卷 / 19期

基金：

中国国家自然科学基金; 新加坡国家研究基金会;

关键词：

bioimaging; fluorogenic probes; glioma; monoamine oxidase A; two-photon fluorescence microscopy; FLUORESCENT-PROBES; MPTP; GENE; OXIDATION; DISEASE;

D O I：

10.1002/anie.202000059

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.

引用

页码：7536 / 7541

页数：6

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