Evidence that a single peptide-MHC complex on a target cell can elicit a cytolytic T cell response

被引：577
作者：
Sykulev, Y [1 ]
Joo, M [1 ]
Vturina, I [1 ]
Tsomides, TJ [1 ]
Eisen, HN [1 ]
机构：
[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
来源：
IMMUNITY | 1996年 / 4卷 / 06期
关键词：
D O I：
10.1016/S1074-7613(00)80483-5
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Using a chemically homogeneous radiolabeled peptide of high specific activity (I-125-QLSPYPFDL, 3.5 x 10(18) cpm per mole) we show that at a peptide concentration (5 pM) causing half-maximal lysis of target cells by a cytolytic T lymphocyte (CTL) clone that recognizes the peptide in association with L(d), a class I MHC protein, only 3 peptide molecules on average are bound by L(d) per target cell. From the distribution of L(d) on the target cells, we suggest that a single peptide-MHC complex per target cell can trigger activation of the T cell cytolytic response.
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收藏
页码：565 / 571
页数：7
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