The Arabidopsis GPI-Anchored LTPg5 Encoded by At3g22600 Has a Role in Resistance against a Diverse Range of Pathogens

被引:11
作者
Ali, Muhammad Amjad [1 ,2 ,3 ]
Abbas, Amjad [1 ,3 ]
Azeem, Farrukh [4 ]
Shahzadi, Mahpara [1 ,5 ]
Bohlmann, Holger [3 ]
机构
[1] Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad 38040, Pakistan
[2] Univ Agr Faisalabad, Ctr Agr Biochem & Biotechnol, Faisalabad 38040, Pakistan
[3] Univ Nat Resources & Life Sci, Dept Crop Sci, Inst Plant Protect, A-1180 Vienna, Austria
[4] GC Univ, Dept Bioinformat & Biotechnol, Plant Biotechnol Lab, Faisalabad 38040, Pakistan
[5] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Grassland Econ & Syst Anal Lab, Lanzhou 730000, Gansu, Peoples R China
基金
奥地利科学基金会;
关键词
lipid transfer protein; antimicrobial peptide; Arabidopsis; Heterodera schachtii; plant defense; LIPID TRANSFER PROTEINS; ANTIMICROBIAL PEPTIDES; BACTERIAL; EXPRESSION; TOLERANCE; FUNGAL; STRESS; BARLEY;
D O I
10.3390/ijms21051774
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arabidopsis contains 34 genes for glycosylphosphatidylinositol (GPI)-anchored LTPg proteins. A motif analysis has placed these into four groups. With one exception, all are produced with a signal peptide and are most likely attached to the cell membrane via the GPI anchor. Several of the LTPg genes across the four groups are downregulated in syncytia induced by the beet cyst nematode Heterodera schachtii. We have here studied At3g22600 encoding LTPg5, which is the most strongly downregulated LTPg gene. It is mainly expressed in roots, and a promoter::GUS line was used to confirm the downregulation in syncytia and also showed downregulation in galls of the root knot nematode Meloidogyne incognita. In contrast, infection with bacteria (Pseudomonas syringae) and fungi (Botrytis cinerea) led to the induction of the gene in leaves. This diverse regulation of LTPg5 indicated a role in resistance, which we confirmed with overexpression lines and a T-DNA mutant. The overexpression lines were more resistant to both nematode species and to P. syringae and B. cinerea, while a knock-out mutant was more susceptible to H. schachtii and P. syringae. Thus, LTPg5 encoded by At3g22600 is part of the Arabidopsis resistance mechanism against pathogens. LTPg5 has probably no direct antimicrobial activity but could perhaps act by associating with a receptor-like kinase, leading to the induction of defense genes such as PR1.
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页数:21
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