Determination of thiamine and its phosphorylated forms in human plasma, erythrocytes and urine by HPLC and fluorescence detection:: a preliminary study on cancer patients

被引：39

作者：

Losa, R

Sierra, MI

Fernández, A

Blanco, D

Buesa, JM

机构：

[1] IUOPA, Hosp Cent Asturias, Lab Med Oncol, Oviedo 33006, Spain

[2] Univ Oviedo, Fac Chem, Dept Phys & Analyt Chem, Oviedo 33006, Spain

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2005年 / 37卷 / 05期

关键词：

ion-pair HPLC; fluorescence detection; thiamine phosphate esters; urine; erythrocytes; plasma; thiamine;

D O I：

10.1016/j.jpba.2004.08.038

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In man, neurotoxicity associated to ifosfamide treatment can be reversed by intravenous thiamine administration. Trying to explain this clinical finding, we decided to study possible changes in thiamine availability and activation inpatients exposed to ifosfamide. Free thiamine and its phosphate esters levels were measured in plasma, erythrocytes and urine by an ion-pair HPLC method with pre-column derivatization, which allowed separation of the fluorescent compounds in less than 10 min. The method was validated by linearity, sensitivity and reproducibility studies, whose values met the demands for bioanalytical assays. This method was applied to assess thiamine status in cancer patients exposed to ifosfamide therapy for advanced disease. © 2004 Elsevier B.V. All rights reserved.

引用

页码：1025 / 1029

页数：5

共 20 条

[1] DETERMINATION OF THIAMIN AND ITS PHOSPHATE-ESTERS IN CULTURED NEURONS AND ASTROCYTES USING AN ION-PAIR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD [J].