Longispora fulva sp. nov., isolated from a forest soil, and emended description of the genus Longispora

A novel actinomycete, strain KZ0017(T), was isolated from a forest soil collected in Ohnuma, Fukushima, Japan. Strain KZ0017(T) formed spore chains borne on top of short sporophores arising from vegetative hyphae. Spores were non-motile and cylindrical with smooth surfaces. Strain KZ0017(T) contained meso-diaminopimelic (A(2)pm) acid, 3-OH A(2)pm, D-glutamic acid, glycine and L-alanine in the cell-wall peptidoglycan, and xylose, mannose, galactose, rhamnose and ribose in cell-wall hydrolysates. The acyl type of the cell-wall polysaccharides was glycolyl. The predominant menaquinones were MK-10(H(4)) and MK-10(H(6)); MK-10(H(8)) was a minor component. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and several unknown lipids and glycolipids. The major fatty acids were iso-C(16:0), 10-methyl-C(17:0) and iso-C(17:1)omega 9c. The DNA G+C content was 70.7 mol%. The 16S rRNA gene sequence of the isolate formed a monophyletic cluster with the single member of the genus Longispora in the family Micromonosporaceae. On the basis of morphological, chemotaxonomic and phylogenetic properties, strain KZ0017(T) represents a novel species of the genus Longispora, for which the name Longispora fulva sp. nov. is proposed; the type strain is KZ0017(T) (=NBRC 105670(T) =DSM 45356(T)).