Possible involvement of the oxLDL/LOX-1 system in the pathogenesis and progression of human intervertebral disc degeneration or herniation

被引：27

作者：

Li, Xinhua ^{[1
]}

Wang, Xuejun ^{[2
]}

Hu, Zhouyang ^{[1
]}

Chen, Zhaoxiong ^{[1
]}

Li, Haoxi ^{[1
]}

Liu, Xiaoming ^{[1
]}

Yong, Zhi Yao ^{[1
]}

Wang, Shanjing ^{[1
]}

Wei, Zhanying ^{[3
]}

Han, Yingchao ^{[1
]}

Tan, Jun ^{[1
]}

Li, Cong ^{[1
]}

He, Xiao Bo ^{[4
]}

Sun, Guixin ^{[5
]}

Wu, Desheng ^{[1
]}

Li, Lijun ^{[1
]}

机构：

[1] Tongji Univ, Sch Med, Dept Spinal Surg, Shanghai East Hosp, 150 JiMo Rd, Shanghai 200120, Peoples R China

[2] Tongji Univ, Sch Med, Dept Cardiovasc Med, Shanghai East Hosp, Shanghai 200120, Peoples R China

[3] Shanghai Jiao Tong Univ, Metab Bone Dis & Genet Res Unit, Div Osteoporosis & Bone Dis, Dept Endocrinol & Metab,Affiliated Peoples Hosp 6, Shanghai, Peoples R China

[4] Tongji Univ, Sch Med, Shanghai East Hosp, Dept Med Imaging, 150 JiMo Rd, Shanghai 200120, Peoples R China

[5] Tongji Univ, Sch Med, Dept Traumatol, Shanghai East Hosp, 150 JiMo Rd, Shanghai 200120, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

LOW-DENSITY-LIPOPROTEIN; LOW-BACK-PAIN; BOVINE ARTICULAR CHONDROCYTES; NUCLEUS PULPOSUS CELLS; LECTIN-LIKE; OXIDIZED LDL; RECEPTOR-1; LOX-1; EXPRESSION; ATHEROSCLEROSIS; BONE;

D O I：

10.1038/s41598-017-07780-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Epidemiological studies have concluded that hyperlipidemia and atherosclerosis were related to intervertebral disc degeneration (IVDD). The presence of oxidized low density lipoprotein (ox-LDL) and the expression of lectin-like oxidized low density lipoprotein receptor 1 (LOX-1) have not been explored in this tissue. In this study, we investigated the presence of ox-LDL and the expression of its receptor LOX-1 in non-degenerated, degenerated or herniated human intervertebral discs (IVDs). The expression of LOX-1 and matrix metalloproteinase 3 (MMP3) were studied after incubating nucleus pulposus cells (NPCs) with ox-LDL. The presence of ox-LDL and LOX-1 was positively related with the extent of IVDD in nucleus pulposus (NP), end-plate cartilage and outer annulus fibrous, but not with the extent of degeneration of inter annulus fibrous. Ox-LDL significantly reduced the viability of human NPCs in a dose and time-dependent manner, and increased the expression of MMP3 induced by LOX-1. Pretreatment with anti-human LOX-1 monoclonal antibody reversed these effects. Ox-LDL, principally mediated by LOX-1, enhanced MMP3 production in NPCs through the NF-kappa B signaling pathway. In conclusion, increased accumulation of ox-LDL and LOX-1 in IVDs indicates a specific role of the receptor-ligand interaction in degeneration or herniation of IVDs.

引用

页数：12

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