Full-field time-resolved fluorescence tomography of small animals

被引：81

作者：

Venugopal, Vivek ^{[1
]}

Chen, Jin ^{[1
]}

Lesage, Frederic ^{[2
,3
]}

Intes, Xavier ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Dept Biomed Engn, Troy, NY 12180 USA

[2] Ecole Polytech Montreal, Dept Genie Elect, Montreal, PQ H3C 3A7, Canada

[3] Ecole Polytech Montreal, Inst Genie Biomed, Montreal, PQ H3C 3A7, Canada

来源：

OPTICS LETTERS | 2010年 / 35卷 / 19期

基金：

加拿大自然科学与工程研究理事会;

关键词：

TURBID MEDIA; ILLUMINATION; LIGHT;

D O I：

10.1364/OL.35.003189

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo-based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model. (C) 2010 Optical Society of America

引用

页码：3189 / 3191

页数：3

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