Analytical validation of commercially available methods for acute phase proteins quantification in pigs

被引：52

作者：

Tecles, F.

Fuentes, P.

Martinez Subiela, S.

Parra, M. D.

Munoz, A.

Ceron, J. J.

机构：

[1] Univ Murcia, Sch Vet, Anim Med & Surg Dept, E-30100 Murcia, Spain

[2] Univ Murcia, Sch Vet, Dept Anim Prod, E-30100 Murcia, Spain

来源：

RESEARCH IN VETERINARY SCIENCE | 2007年 / 83卷 / 01期

关键词：

acute phase proteins; porcine; validation; haptoglobin; C-reactive protein; serum amyloid A; porcine major acute phase protein;

D O I：

10.1016/j.rvsc.2006.10.005

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to validate commercially available methods for porcine haptoglobin (Hp), C-reactive protein (CRP), serum amyloid A (SAA) and major acute phase protein (Pig-MAP) determinations. Intra and inter assay coefficients of variation (CVs) were lower than 20% in all cases with exception of inter assay CVs for CRP and Pig-MAP assays with samples of low acute phase proteins concentration, and for SAA assay at any acute phase proteins concentration. All methods showed good linearity and detection limits were low enough to detect APPs levels in healthy animals. Hp and SAA were very affected by haemolysis. Lipaemia influenced mainly on SAA determination. Over 15-fold increase was observed in CRP and SAA concentrations after artificially induced inflammation by a single subcutaneous dose of turpentine, whereas Hp and Pig-MAP increased less than 5-fold. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：133 / 139

页数：7

共 21 条

[1]

ALLEMAN AR, 1990, VET MED-US, V85, P1272

[2]

[Anonymous], 2001, GUID IND BIOAN METH

[3] Elevated serum haptoglobin in pigs infected with porcine reproductive and respiratory syndrome virus [J].