Influence of long-term fertilization on soil microbial biomass, dehydrogenase activity, and bacterial and fungal community structure in a brown soil of northeast China

被引:151
作者
Luo, Peiyu [1 ,2 ]
Han, Xiaori [2 ]
Wang, Yan [3 ]
Han, Mei [1 ,2 ]
Shi, Hui [1 ,2 ]
Liu, Ning [1 ]
Bai, Hongzhi [1 ,2 ]
机构
[1] Shenyang Agr Univ, Coll Land & Environm, Shenyang 110866, Liaoning, Peoples R China
[2] Natl Engn Lab Efficient Utilizat Soil & Fertilize, Shenyang 110866, Liaoning, Peoples R China
[3] Shenyang Agr Univ, Foreign Language Teaching Dept, Shenyang 110866, Liaoning, Peoples R China
关键词
Long-term fertilization; Bacterial and fungal community structure; Soil microbial biomass carbon; Dehydrogenase activity; PCR-DGGE; PHOSPHORUS FERTILIZATION; BIOLOGICAL-PROPERTIES; MANURE APPLICATION; ENZYME-ACTIVITIES; ORGANIC-CARBON; DIVERSITY; RESIDUES; ROTATION; TILLAGE; MAIZE;
D O I
10.1007/s13213-014-0889-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study, the effect of mineral fertilizer and organic manure were evaluated on soil microbial biomass, dehydrogenase activity, bacterial and fungal community structure in a long-term (33 years) field experiment. Except for the mineral nitrogen fertilizer (N) treatment, long-term fertilization greatly increased soil microbial biomass carbon (SMBC) and dehydrogenase activity. Organic manure had a significantly greater impact on SMBC and dehydrogenase activity, compared with mineral fertilizers. Bacterial and fungal community structure was analyzed by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Long-term fertilization increased bacterial and fungal ribotype diversity. Total soil nitrogen (TN) and phosphorus (TP), soil organic carbon (SOC) and available phosphorus (AP) had a similar level of influence on bacterial ribotypes while TN, SOC and AP had a larger influence than alkali-hydrolyzable nitrogen (AHN) on fungal ribotypes. Our results suggested that long-term P-deficiency fertilization can significantly decrease soil microbial biomass, dehydrogenase activity and bacterial diversity. N-fertilizer and SOC have an important influence on bacterial and fungal communities.
引用
收藏
页码:533 / 542
页数:10
相关论文
共 49 条
[1]   RATIOS OF MICROBIAL BIOMASS CARBON TO TOTAL ORGANIC-CARBON IN ARABLE SOILS [J].
ANDERSON, TH ;
DOMSCH, KH .
SOIL BIOLOGY & BIOCHEMISTRY, 1989, 21 (04) :471-479
[2]  
[Anonymous], 1958, SOIL CHEM ANAL
[3]  
Aref S, 1998, ADV AGRON, V62, P153
[4]   Long-Term Phosphorus Fertilization Impacts Soil Fungal and Bacterial Diversity but not AM Fungal Community in Alfalfa [J].
Beauregard, M. S. ;
Hamel, C. ;
Nayyar, Atul ;
St-Arnaud, M. .
MICROBIAL ECOLOGY, 2010, 59 (02) :379-389
[5]   Long-term organic farming fosters below and aboveground biota:: Implications for soil quality, biological control and productivity [J].
Birkhofer, Klaus ;
Bezemer, T. Martijn ;
Bloem, Jaap ;
Bonkowski, Michael ;
Christensen, Soren ;
Dubois, David ;
Ekelund, Fleming ;
Fliessbach, Andreas ;
Gunst, Lucie ;
Hedlund, Katarina ;
Maeder, Paul ;
Mikola, Juha ;
Robin, Christophe ;
Setala, Heikki ;
Tatin-Froux, Fabienne ;
Van der Putten, Wim H. ;
Scheu, Stefan .
SOIL BIOLOGY & BIOCHEMISTRY, 2008, 40 (09) :2297-2308
[6]  
Bremner J. M., 1982, Methods of soil analysis. Part 2. Chemical and microbiological properties, P595
[7]   Root exudates regulate soil fungal community composition and diversty [J].
Broeckling, Corey D. ;
Broz, Amanda K. ;
Bergelson, Joy ;
Manter, Daniel K. ;
Vivanco, Jorge M. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (03) :738-744
[8]  
CARSON PL, 1980, N DAK AGR EXP STA BU, P17
[9]  
CASIDA L. E., 1964, SOIL SCI, V98, P371, DOI 10.1097/00010694-196412000-00004
[10]   Soil microbial biomass, dehydrogenase activity, bacterial community structure in response to long-term fertilizer management [J].
Chu, Haiyan ;
Lin, Xiangui ;
Fujii, Takeshi ;
Morimoto, Sho ;
Yagi, Kazuyuki ;
Hu, Junli ;
Zhang, Jiabao .
SOIL BIOLOGY & BIOCHEMISTRY, 2007, 39 (11) :2971-2976