Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth

被引:174
作者
Lee, Yong Jik [1 ,2 ]
Szumlanski, Amy [3 ]
Nielsen, Erik [3 ]
Yang, Zhenbiao [1 ,2 ]
机构
[1] Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA
[2] Univ Calif Riverside, Ctr Plant Cell Biol, Inst Integrat Genome Biol, Riverside, CA 92521 USA
[3] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA
关键词
D O I
10.1083/jcb.200801086
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
he dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis.
引用
收藏
页码:1155 / 1168
页数:14
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