Channel thickness variations could degrade resolution in electrophoresis

被引：1
作者：
Holzwarth, G
机构：
[1] Department of Physics, Wake Forest University, Winston-Salem, NC
[2] Department of Physics, Wake Forest University, Winston-Salem, NC 27109
来源：
ELECTROPHORESIS | 1996年 / 17卷 / 10期
关键词：
capillary electrophoresis; channel width; channel fluctuations; resolution; bandwidth;
D O I：
10.1002/elps.1150171016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Variations in the lateral dimensions of capillaries and ultrathin gels along their length could lead to a distortion of the sample zone because charged particles traveling near the wall must travel a longer distance under a smaller field than particles traveling along the centerline. The size of the effect is computed by solving Laplace's equation for the potential everywhere in a conducting slab with a discontinuity in the lateral dimensions and integrating the travel time along different streamlines.
引用
收藏
页码：1587 / 1589
页数：3
相关论文
共 11 条
[1]   PROBING THE INNER SURFACE OF A CAPILLARY WITH THE ATOMIC-FORCE MICROSCOPE [J].
BARBERI, R ;
GIOCONDO, M ;
BARTOLINO, R ;
RIGHETTI, PG .
ELECTROPHORESIS, 1995, 16 (08) :1445-1450
[2]  
Grossman P.D., 1992, CAPILLARY ELECTROPHO
[3]   DIODE-ARRAY DETECTION IN CAPILLARY ELECTROPHORESIS [J].
HEIGER, DN ;
KALTENBACH, P ;
SIEVERT, HJP .
ELECTROPHORESIS, 1994, 15 (10) :1234-1247
[4]  
Ivanov V.I., 1995, Handbook of Conformal Mapping with Computer-Aided Visualization
[5]   CONTRIBUTION OF CAPILLARY COILING TO ZONE DISPERSION IN CAPILLARY ZONE ELECTROPHORESIS [J].
KASICKA, V ;
PRUSIK, Z ;
GAS, B ;
STEDRY, M .
ELECTROPHORESIS, 1995, 16 (11) :2034-2038
[6]   ANALYSIS OF RESTRICTION FRAGMENTS OF T7 DNA AND DETERMINATION OF MOLECULAR-WEIGHTS BY ELECTROPHORESIS IN NEUTRAL AND ALKALINE GELS [J].
MCDONELL, MW ;
SIMON, MN ;
STUDIER, FW .
JOURNAL OF MOLECULAR BIOLOGY, 1977, 110 (01) :119-146
[7]   OPTICAL IMPROVEMENTS OF A Z-SHAPED CELL FOR HIGH-SENSITIVITY UV ABSORBENCY DETECTION IN CAPILLARY ELECTROPHORESIS [J].
MORING, SE ;
REEL, RT ;
VANSOEST, REJ .
ANALYTICAL CHEMISTRY, 1993, 65 (23) :3454-3459
[8]   DIFFUSION, JOULE HEATING, AND BAND BROADENING IN CAPILLARY GEL-ELECTROPHORESIS OF DNA [J].
SLATER, GW ;
MAYER, P ;
GROSSMAN, PD .
ELECTROPHORESIS, 1995, 16 (01) :75-83
[9]   ELECTROPHORETIC RESOLUTION VERSUS FLUCTUATIONS OF THE LATERAL DIMENSIONS OF A CAPILLARY [J].
SLATER, GW ;
MAYER, P .
ELECTROPHORESIS, 1995, 16 (05) :771-779
[10]  
SMYTHE WR, 1950, STGATIC DYNAMIC ELEC, P235
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