Cryo-EM-the first thirty years

被引：90

作者：

Dubochet, J. ^{[1
]}

机构：

[1] Univ Lausanne, Dept Ecol & Evolut, Biophore, Fac Biol & Med,DEE, CH-1015 Lausanne, Switzerland

来源：

JOURNAL OF MICROSCOPY | 2012年 / 245卷 / 03期

关键词：

CEMOVIS; Cryo-electron microscopy; Vitreous section; Vitrification; Water; FROZEN HYDRATED SECTIONS; ELECTRON-MICROSCOPY; CRYOELECTRON MICROSCOPY; VITREOUS SECTIONS; BIOLOGICAL SPECIMENS; MOLECULAR-STRUCTURE; AQUEOUS-SOLUTIONS; OUTER-MEMBRANE; TOMOGRAPHY; ARCHITECTURE;

D O I：

10.1111/j.1365-2818.2011.03569.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Thirty years ago, in December 1981, The Journal of Microscopy published a very short paper entitled Vitrification of pure water for electron microscopy. It turned out to be important for the development of cryo-electron microscopy and it contributed to reverse, from foe to friend, the status of water in electron microscopists minds. This change has brought obvious gains. The future will tell how many more are still to come.

引用

页码：221 / 224

页数：4

共 43 条

[1] CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].

ADRIAN, M ;

DUBOCHET, J ;

LEPAULT, J ;

MCDOWALL, AW .

NATURE, 1984, 308 (5954) :32-36

[2] Cryo-electron microscopy of vitreous sections [J].

Al-Amoudi, A ;

Chang, JJ ;

Leforestier, A ;

McDowall, A ;

Salamin, LM ;

Norlén, LPO ;

Richter, K ;

Blanc, NS ;

Studer, D ;

Dubochet, J .

EMBO JOURNAL, 2004, 23 (18) :3583-3588

[3] The molecular architecture of cadherins in native epidermal desmosomes [J].

Al-Amoudi, Ashraf ;

Diez, Daniel Castano ;

Betts, Matthew J. ;

Frangakis, Achilleas S. .

NATURE, 2007, 450 (7171) :832-U8

[4] The three-dimensional molecular structure of the desmosomal plaque [J].

Al-Amoudi, Ashraf ;

Castano-Diez, Daniel ;

Devos, Damien P. ;

Russell, Robert B. ;

Johnson, Graham T. ;

Frangakis, Achilleas S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (16) :6480-6485

[5] Comparison of different methods for thin section EM analysis of Mycobacterium smegmatis [J].

Bleck, C. K. E. ;

Merz, A. ;

Gutierrez, M. G. ;

Walther, P. ;

Dubochet, J. ;

Zuber, B. ;

Griffiths, G. .

JOURNAL OF MICROSCOPY, 2010, 237 (01) :23-38

[6] Golgi apparatus studied in vitreous sections [J].

Bouchet-Marquis, C. ;

Starkuviene, V. ;

Grabenbauer, M. .

JOURNAL OF MICROSCOPY, 2008, 230 (02) :308-316

[7] Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture [J].

Bouchet-Marquis, C ;

Dubochet, J ;

Fakan, S .

HISTOCHEMISTRY AND CELL BIOLOGY, 2006, 125 (1-2) :43-51

[8] COMPLETE VITRIFICATION IN PURE LIQUID WATER AND DILUTE AQUEOUS-SOLUTIONS [J].

BRUGGELLER, P ;

MAYER, E .

NATURE, 1980, 288 (5791) :569-571

[9] FREEZING, SECTIONING AND OBSERVATION ARTIFACTS OF FROZEN HYDRATED SECTIONS FOR ELECTRON-MICROSCOPY [J].

CHANG, JJ ;

MCDOWALL, AW ;

LEPAULT, J ;

FREEMAN, R ;

WALTER, CA ;

DUBOCHET, J .

JOURNAL OF MICROSCOPY, 1983, 132 (OCT) :109-123

[10] CEMOVIS on a pathogen: analysis of Bacillus anthracis spores [J].

Couture-Tosi, Evelyne ;

Ranck, Jean-Luc ;

Haustant, Georges ;

Pehau-Arnaudet, Gerard ;

Sachse, Martin .

BIOLOGY OF THE CELL, 2010, 102 (11) :609-619

← 1 2 3 4 5 →