Acid protease purification by dye affinity chromatography

被引：0
|
作者：
del Cañizo, AAN [1 ]
Lahore, HMF [1 ]
Miranda, MV [1 ]
Cascone, O [1 ]
机构：
[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Microbiol Ind & Biotecnol, RA-1113 Buenos Aires, DF, Argentina
来源：
AFINIDAD | 2001年 / 58卷 / 493期
关键词：
acid protease; purification; affinity chromatography; triazinic dyes;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
After screening of 9 immobilised triazinic dyes, Red F5B was selected as the best ligand for chymosin and Mucor miehei acid protease purification, at pHs 6.5 and 5.4 respectively. For Mucor bacilliformis protease purification from a crude extract of a solid-state culture, Red HE-3B was selected, allowing a 95.2% recovery of pure enzyme.
引用
收藏
页码：231 / 233
页数：3
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