A simple, sensitive and reproducible assay for pyridoxal 5′-phosphate and 4-pyridoxic acid in human plasma

被引：94

作者：

Bates, CJ ^{[1
]}

Pentieva, KD ^{[1
]}

Matthews, N ^{[1
]}

Macdonald, A ^{[1
]}

机构：

[1] MRC, Cambridge CB4 1XJ, England

来源：

CLINICA CHIMICA ACTA | 1999年 / 280卷 / 1-2期

关键词：

vitamin B-6 status; high performance liquid chromatography; plasma pyridoxal 5 '-phosphate; plasma 4-pyridoxic acid; older adults; National Diet and Nutrition Survey;

D O I：

10.1016/S0009-8981(98)00173-9

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We describe a procedure for the measurement of pyridoxal 5'-phosphate and of 4-pyridoxic acid in human plasma samples. It is based on the conversion of pyridoxal 5'-phosphate to 4-pyridoxic acid 5'-phosphate by cyanide in alkaline medium, followed by a high pressure liquid chromatographic separation, with fluorescence detection at acid pH. The assay is robust, sensitive, linear over a wide range, reproducible, and simple to perform. Samples stored at -80 degrees C are stable. Satisfactory agreement was obtained with results from the tyrosine decarboxylase-based assay for pyridoxal 5'-phosphate, in two other laboratories. Plasma samples from a National Survey of older British people were analyzed, and reference intervals for plasma pyridoxal 5'-phosphate intervals were derived. From the lower 2.5 percentile of the reference group, taken as the lower cut-off of the normal range, ca. 20% of elderly men and 11% of elderly women in the UK showed evidence of biochemical deficiency. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：101 / 111

页数：11

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