Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry

被引：58

作者：

Aknoun, Sherazade ^{[1
,2
]}

Bon, Pierre ^{[1
]}

Savatier, Julien ^{[1
]}

Wattellier, Benoit ^{[2
]}

Monneret, Serge ^{[1
]}

机构：

[1] Aix Marseille Univ, CNRS, Inst Fresnel, UMR 7249, Campus St Jerome, F-13013 Marseille, France

[2] PHASICS SA, F-91128 Palaiseau, France

来源：

OPTICS EXPRESS | 2015年 / 23卷 / 12期

关键词：

DIGITAL HOLOGRAPHIC MICROSCOPY; OPTICAL COHERENCE TOMOGRAPHY; POLARIZED PHASE MICROSCOPY; REFRACTIVE-INDEX; LIVING CELLS; COLLAGEN; BIREFRINGENCE; TRANSPARENT; RESOLUTION; MATRICES;

D O I：

10.1364/OE.23.016383

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system combines quantitative phase images with varying polarization excitation to create retardance images. This technique is compatible with living samples and gives information about the local retardance and structure of their anisotropic components. We applied our approach to collagen fibers leading to a birefringence value of (3.4 +/- 0.3) . 10(-3) and to living cells, showing that cytoskeleton can be imaged label-free. (C) 2015 Optical Society of America

引用

页码：16383 / 16406

页数：24

共 39 条

[31] Diffraction phase microscopy for quantifying cell structure and dynamics [J].

Popescu, G ;

Ikeda, T ;

Dasari, RR ;

Feld, MS .

OPTICS LETTERS, 2006, 31 (06) :775-777

[32] Extended Hartmann test based on the pseudoguiding property of a Hartmann mask completed by a phase chessboard [J].

Primot, J ;

Guérineau, N .

APPLIED OPTICS, 2000, 39 (31) :5715-5720

[33] Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy [J].

Rappaz, B ;

Marquet, P ;

Cuche, E ;

Emery, Y ;

Depeursinge, C ;

Magistretti, PJ .

OPTICS EXPRESS, 2005, 13 (23) :9361-9373

[34] Lifeact: a versatile marker to visualize F-actin [J].

Riedl, Julia ;

Crevenna, Alvaro H. ;

Kessenbrock, Kai ;

Yu, Jerry Haochen ;

Neukirchen, Dorothee ;

Bista, Michal ;

Bradke, Frank ;

Jenne, Dieter ;

Holak, Tad A. ;

Werb, Zena ;

Sixt, Michael ;

Wedlich-Soldner, Roland .

NATURE METHODS, 2008, 5 (07) :605-607

[35] New, simple theory-based, accurate polarization microscope for birefringence imaging of biological cells [J].

Shin, In Hee ;

Shin, Sang-Mo ;

Kim, Dug Young .

JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (01)

[36] Quantitative measurement of the distribution and alignment of collagen fibers in unfixed aortic tissues [J].

Sugita, Shukei ;

Matsumoto, Takeo .

JOURNAL OF BIOMECHANICS, 2013, 46 (07) :1403-1407

[37] Single-shot polarization-imaging digital holography based on simultaneous phase-shifting interferometry [J].

Tahara, Tatsuki ;

Awatsuji, Yasuhiro ;

Shimozato, Yuki ;

Kakue, Takashi ;

Nishio, Kenzo ;

Ura, Shogo ;

Kubota, Toshihiro ;

Matoba, Osamu .

OPTICS LETTERS, 2011, 36 (16) :3254-3256

[38] Jones phase microscopy of transparent and anisotropic samples [J].

Wang, Zhuo ;

Millet, Larry J. ;

Gillette, Martha U. ;

Popescu, Gabriel .

OPTICS LETTERS, 2008, 33 (11) :1270-1272

[39] Live-cell mass profiling: an emerging approach in quantitative biophysics [J].

Zangle, Thomas A. ;

Teitell, Michael A. .

NATURE METHODS, 2014, 11 (12) :1221-1228

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