α-amylase production from catabolite derepressed Bacillus subtilis KCC103 utilizing sugarcane bagasse hydrolysate

被引：127

作者：

Rajagopalan, Gobinath ^{[1
]}

Krishnan, Chandraraj ^{[1
]}

机构：

[1] Indian Inst Technol Madras, Dept Biotechnol, Madras 600036, Tamil Nadu, India

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 08期

关键词：

alpha-amylase; Bacillus subtilis; sugarcane bagasse hydrolysate; catabolite repression; response surface methodology;

D O I：

10.1016/j.biortech.2007.06.001

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A catabolite derepressed Bacillus subtilis strain KCC103 was used to produce a-amylase in medium containing sugarcane bagasse hydrolysate (SBH). Addition of SBH (1% reducing sugar (w/v)) to the nutrient medium supported maximum alpha-amylase production of 67.4 U ml(-1). HPLC analysis of SBH showed the presence of glucose, xylose and arabinose in the ratio of 0.9:1.0:0.16 (w/w/w). In SBH-medium glucose and xylose were consumed completely while arabinose remained unutilized. Uptake rate of glucose was 2-folds higher than xylose but rate of alpha-amylase production with xylose was 1.5-folds higher than glucose. Arabinose had no effect on growth and a-amylase synthesis. Further, alpha-amylase production in SBH-medium was enhanced to 144.5 U ml(-1) (2.2-fold) by response surface methodology where the levels of SBH, and other media components were varied. The modified medium consisted of (in g l(-1)) SBH: 24; peptone: 17.43; yeast extract: 1.32 and beef extract: 1.82. High level of SBH showed no significant inhibition of alpha-amylase synthesis. The derepressed strain KCC103 is useful to produce alpha-amylase economically in short time (30-36 h). (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：3044 / 3050

页数：7