Online coupling of molecularly imprinted solid-phase extraction to HPLC for determination of trace tetracycline antibiotic residues in egg samples

被引：32
作者：
Jing, Tao [1 ]
Niu, Jiwei [1 ]
Xia, Huan [1 ]
Dai, Qing [1 ]
Zheng, Hongyan [1 ]
Hao, Qiaolin [1 ]
Mei, Surong [1 ]
Zhou, Yikai [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, MOE Key Lab Environm & Hlth,Inst Environm Med, Wuhan 430030, Hubei, Peoples R China
来源：
JOURNAL OF SEPARATION SCIENCE | 2011年 / 34卷 / 12期
关键词：
Online solid-phase extraction; Molecularly imprinted polymers; Tetracycline antibiotics; Egg samples; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; POLYMERS; WATER; OXYTETRACYCLINE; CHEMILUMINESCENCE; SYSTEM; MUSCLE; HONEY; UV;
D O I：
10.1002/jssc.201100030
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An automated system has been developed for the determination of trace tetracycline antibiotics (TCs) in egg samples, based on online molecularly imprinted solid-phase extraction (MISPE) coupling with high-performance liquid chromatography (HPLC). Oxytetracycline and chlortetracycline were chosen as mixed templates to synthesize highly selective molecularly imprinted polymers for online extraction. Under the optimal online MISPE-HPLC condition, 10 mL egg samples were injected into the MISPE column and then the matrix was washed out. By rotating the switching valve, TCs were transferred to the analytical column and then separated by HPLC. Because sample pretreatment and chromatographic separation were carried out simultaneously, the whole analytical time (18 min) was significantly shortened compared with conventional offline techniques. The detection limits ranged from 0.8 to 1.3 ng/g. The enhancement factors were in the range of 159-410. The spiked recoveries of TCs in real egg samples ranged from 91.6 to 107.6% and the relative standard deviations (RSDs) were not higher than 4.0%.
引用
收藏
页码：1469 / 1476
页数：8
相关论文
共 31 条
[1]   Composting rapidly reduces levels of extractable oxytetracycline in manure from therapeutically treated beef calves [J].
Arikan, Osman A. ;
Sikora, Lawrence J. ;
Mulbry, Walter ;
Khan, Shahamat U. ;
Foster, Gregory D. .
BIORESOURCE TECHNOLOGY, 2007, 98 (01) :169-176
[2]   An original approach to determining traces of tetracycline antibiotics in milk and eggs by solid-phase extraction and liquid chromatography/mass spectrometry [J].
Bruno, F ;
Curini, R ;
Di Corcia, A ;
Nazzari, M ;
Pallagrosi, M .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (14) :1365-1376
[3]   Molecularly-imprinted polymers selective for tetracycline binding [J].
Cai, WS ;
Gupta, RB .
SEPARATION AND PURIFICATION TECHNOLOGY, 2004, 35 (03) :215-221
[4]   Synthesis and application of an oxytetracycline imprinted polymer for the solid-phase extraction of tetracycline antibiotics [J].
Caro, E ;
Marcé, RM ;
Cormack, PAG ;
Sherrington, DC ;
Borrull, F .
ANALYTICA CHIMICA ACTA, 2005, 552 (1-2) :81-86
[5]   Preparation of magnetic molecularly imprinted polymer for the separation of tetracycline antibiotics from egg and tissue samples [J].
Chen, Ligang ;
Liu, Jun ;
Zeng, Qinglei ;
Wang, Hui ;
Yu, Aimin ;
Zhang, Hanqi ;
Ding, Lan .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (18) :3710-3719
[6]  
CHINESE NATIONAL STANDARD, 2007, 213172007 GB CHIN NA
[7]   Simplified liquid-chromatographic determination of residues of tetracycline antibiotics in eggs [J].
Furusawa, N .
CHROMATOGRAPHIA, 2001, 53 (1-2) :47-50
[8]   Quantitative measurements of corticosteroids in ex vivo samples using on-line SPE-LC/MS/MS [J].
Gao, Lan ;
Chiou, William J. ;
Camp, Heidi S. ;
Burns, David J. ;
Cheng, Xueheng .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (03) :303-310
[9]   Rapid and sensitive determination of polycyclic aromatic hydrocarbons in atmospheric particulates using fast high-performance liquid chromatography with on-line enrichment system [J].
Hashi, Yuki ;
Wang, Tian-Ran ;
Du, Wei ;
Lin, Jin-Ming .
TALANTA, 2008, 74 (04) :986-991
[10]   Molecularly imprinted polymer online solid-phase extraction coupled with high-performance liquid chromatography-UV for the determination of three Sulfonamides in pork and chicken [J].
He, Jinxing ;
Wang, Shuo ;
Fang, Guozhen ;
Zhu, Huaping ;
Zhang, Yan .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (09) :2919-2925
1234