Role of σ-factor (orf21) in clavulanic acid production in Streptomyces clavuligerus NRRL3585

被引：12

作者：

Jnawali, Hum Nath ^{[1
]}

Liou, Kwangkyoung ^{[1
]}

Sohng, Jae Kyung ^{[1
]}

机构：

[1] SunMoon Univ, iBR, Dept Pharmaceut Engn, Asan 336708, Chungnam, South Korea

来源：

MICROBIOLOGICAL RESEARCH | 2011年 / 166卷 / 05期

关键词：

Clavulanic acid; Replication; Sigma factor; Streptomyces clavuligerus NRRL3585; MYCOBACTERIUM-TUBERCULOSIS; CLAVAMINATE SYNTHASE; TRANSCRIPTION; BIOSYNTHESIS; GENE; PROTEIN; DIFFERENTIATION; IDENTIFICATION; INTERMEDIATE; DELETION;

D O I：

10.1016/j.micres.2010.07.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A putative sigma factor gene, orf21, was disrupted or overexpressed in the wild-type clavulanic acid (CA) producer Streptomyces clavuligerus NRRL3585 and characterized. An orf21 mutant (Streptomyces clavuligerus HN14) of S. clavuligerus was obtained by insertional inactivation via double-crossover. Although there was little reduction of sporulation in the mutant, the growth pattern was similar between mutant and wild-type. The production was reduced by 10-15% in S. clavuligerus HN14 compared to that in wild-type. Overexpression of orf21 in wild-type cells caused hyperproduction of spores on solid medium and increased clavulanic acid production by 1.43-fold. The overexpression of orf21 in wild-type S. clavuligerus stimulated the expression of the early clavulanic acid genes, ceas2 and cas2, and the regulatory gene, ccaR, as demonstrated by RT-PCR. The elevation of the ceas2, cas2 and ccaR transcripts was consistent with the enhanced production of clavulanic acid. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：369 / 379

页数：11

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