Estimation of nuclear DNA content in populations of Atriplex halimus L. (Chenopodiaceae) by flow cytometry

被引:0
|
作者
Kheiria, Hcini [1 ]
Walker, David J. [2 ]
Correal, Enrique [2 ]
Bouzid, Sadok [1 ]
机构
[1] Fac Sci Tunis, Dept Biol Sci, El Manar II Tunis 2092, Tunisia
[2] IMIDA, Dept Recursos Nat & Desarrollo Rural, Murcia 30150, Spain
关键词
Atriplex halimus L; flow cytometry; nuclear DNA content;
D O I
10.1080/00087114.2008.10589624
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Atriplex halimus L. is a perennial native shrub of the Mediterranean Basin with an excellent tolerance to drought and salinity. The aim of this work was to estimate nuclear DNA content in populations of A. halimus L. by flow cytometry, an efficient method to estimate ploidy levels and to determine nuclear DNA content in plants. Flow cytometry is a technique which permits rapid estimation of nuclear DNA content. Because of its speed, precision and convenience, this method of analysis of nuclear DNA content finds an enormous number of applications which cover basic research, breeding and production. The results obtained indicate that the technique might greatly simplify the analysis of plant genomes at the molecular level.
引用
收藏
页码:160 / 163
页数:4
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