Estimation of nuclear DNA content in populations of Atriplex halimus L. (Chenopodiaceae) by flow cytometry

被引：0
|
作者：
Kheiria, Hcini [1 ]
Walker, David J. [2 ]
Correal, Enrique [2 ]
Bouzid, Sadok [1 ]
机构：
[1] Fac Sci Tunis, Dept Biol Sci, El Manar II Tunis 2092, Tunisia
[2] IMIDA, Dept Recursos Nat & Desarrollo Rural, Murcia 30150, Spain
来源：
CARYOLOGIA | 2008年 / 61卷 / 02期
关键词：
Atriplex halimus L; flow cytometry; nuclear DNA content;
D O I：
10.1080/00087114.2008.10589624
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Atriplex halimus L. is a perennial native shrub of the Mediterranean Basin with an excellent tolerance to drought and salinity. The aim of this work was to estimate nuclear DNA content in populations of A. halimus L. by flow cytometry, an efficient method to estimate ploidy levels and to determine nuclear DNA content in plants. Flow cytometry is a technique which permits rapid estimation of nuclear DNA content. Because of its speed, precision and convenience, this method of analysis of nuclear DNA content finds an enormous number of applications which cover basic research, breeding and production. The results obtained indicate that the technique might greatly simplify the analysis of plant genomes at the molecular level.
引用
收藏
页码：160 / 163
页数：4
相关论文
共 50 条
  • [1] Determination of ploidy and nuclear DNA content in populations of Atriplex halimus (Chenopodiaceae)
    Walker, DJ
    Moñino, I
    González, E
    Frayssinet, N
    Correal, E
    BOTANICAL JOURNAL OF THE LINNEAN SOCIETY, 2005, 147 (04) : 441 - 448
  • [2] Polygamy in Atriplex halimus L. (Chenopodiaceae)
    Talamali, A
    Dutuit, P
    Le Thomas, A
    Gorenflot, R
    COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 2001, 324 (02): : 107 - 113
  • [3] Chromosome numbers in Tunisian populations of Atriplex halimus L. (Chenopodiaceae)
    El Ferchichi Ouarda, Hela
    H'cini, Khiria
    Bouzid, Sadok
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2006, 5 (12): : 1190 - 1193
  • [4] Determination of Ploidy Level and Nuclear DNA Content in Tunisian Populations of Atriplex halimus L.
    Kheiria Hcini
    David J. Walker
    Sadok Bouzid
    Elena González
    Nora Frayssinet
    Enrique Correal
    Genetic Resources and Crop Evolution, 2006, 53 : 1 - 5
  • [5] Determination of ploidy level and nuclear DNA content in Tunisian populations of Atriplex halimus L.
    Hcini, K
    Walker, DJ
    Bouzid, S
    González, E
    Frayssinet, N
    Correal, E
    GENETIC RESOURCES AND CROP EVOLUTION, 2006, 53 (01) : 1 - 5
  • [6] Morphological variability of fruit and chromosome numbers in Tunisian populations of Atriplex halimus L. (Chenopodiaceae)
    Hcini, Kheiria
    Ouerda, Hela Ferchichi
    Bouzid, Sadok
    CARYOLOGIA, 2007, 60 (03) : 203 - 211
  • [7] Micropropagation of Atriplex halimus L.
    Papafotiou, M.
    Martinis, A. N.
    Bertsouklis, K. F.
    Majumder, D. A. N.
    XXIX INTERNATIONAL HORTICULTURAL CONGRESS ON HORTICULTURE: SUSTAINING LIVES, LIVELIHOODS AND LANDSCAPES (IHC2014): INTERNATIONAL SYMPOSIUM ON MICROPROPAGATION AND IN VITRO TECHNIQUES, 2016, 1113 : 207 - 210
  • [8] Reproductive phenology and pre-dispersal fruit predation in Atriplex halimus L. (Chenopodiaceae)
    Romera, Prado
    Fernandez-Illescas, Francisca
    Nieva, F. Javier J.
    Rodriguez-Rubio, Pilar
    Sanchez-Gullon, Enrique
    Munoz-Rodriguez, Adolfo F.
    BOTANICAL STUDIES, 2013, 54
  • [9] Reproductive phenology and pre-dispersal fruit predation in Atriplex halimus L. (Chenopodiaceae)
    Prado Romera
    Francisca Fernández-Illescas
    F Javier J Nieva
    Pilar Rodríguez-Rubio
    Enrique Sánchez-Gullón
    Adolfo F Muñoz-Rodríguez
    Botanical Studies, 54
  • [10] The phenological study of Atriplex halimus L.
    Abbad, A
    Benchaabane, A
    AFRICAN JOURNAL OF ECOLOGY, 2004, 42 (01) : 69 - 73
12345