Reusable chromium-coated quartz crystal microbalance for immunosensing

被引：12
作者：
Oliver, M. J. [1 ]
Hernando-Garcia, J. [1 ]
Pobedinskas, P. [2 ]
Haenen, K. [2 ,3 ]
Rios, A. [4 ]
Sanchez-Rojas, J. L. [1 ]
机构：
[1] Univ Castilla La Mancha, Dept Ingn Elect Elect Automat & Comunicac, ETSI Ind, E-13071 Ciudad Real, Spain
[2] Hasselt Univ, IMO, B-3590 Diepenbeek, Belgium
[3] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium
[4] Univ Castilla La Mancha, Dept Quim Analit & Tecnol Alimentos, Fac Quim, E-13071 Ciudad Real, Spain
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 88卷 / 01期
关键词：
Oxidized chromium; Immunosensors; Quartz crystal microbalance; SURFACE-PLASMON RESONANCE; SELF-ASSEMBLED MONOLAYERS; ANTIBODY IMMOBILIZATION; VISCOELASTIC PROPERTIES; ADSORPTION; PROTEIN; GOLD; DISSIPATION; BIOSENSOR; KINETICS;
D O I：
10.1016/j.colsurfb.2011.06.030
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The application of oxidized chromium as a reusable platform for the development of immunosensors is presented. Chromium films were deposited on quartz crystal microbalances to study the affinity interaction between rabbit immunoglobulin G (IgG) and goat anti-rabbit IgG. A covalent approach, based on the silane chemistry, was followed for the grafting of either the rabbit IgG or the anti-rabbit IgG on the silane-modified chromium surface. Next the differences between the deposition of rabbit IgG on immobilized anti-rabbit IgG and the deposition of anti-rabbit IgG on immobilized rabbit IgG were investigated. The chromium layer could be reused between experiments, after the proper removal of the organic layers with piranha etch, obtaining a high repeatability in the steps of the functionalization protocol. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：191 / 195
页数：5
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