Microbial structures and community functions of anaerobic sludge in six full-scale wastewater treatment plants as revealed by 454 high-throughput pyrosequencing

被引：272
作者：
Shu, Duntao [1 ,2 ]
He, Yanling [3 ]
Yue, Hong [4 ,5 ]
Wang, Qingyi [6 ]
机构：
[1] Xi An Jiao Tong Univ, Ctr Mitochondrial Biol & Med, Key Lab Biomed Informat Engn, Minist Educ,Sch Life Sci & Technol, Xian 710049, Shaanxi, Peoples R China
[2] Xi An Jiao Tong Univ, Frontier Inst Sci & Technol, Xian 710049, Shaanxi, Peoples R China
[3] Xi An Jiao Tong Univ, Sch Human Settlements & Civil Engn, Xian 710049, Shaanxi, Peoples R China
[4] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Coll Agron, China Wheat Improvement Ctr, Yangling 712100, Shaanxi, Peoples R China
[5] Northwest A&F Univ, Yangling Branch, China Wheat Improvement Ctr, Yangling 712100, Shaanxi, Peoples R China
[6] Xi An Jiao Tong Univ, Sch Chem Engn & Technol, Xian 710049, Shaanxi, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2015年 / 186卷
关键词：
Anaerobic sludge; Pyrosequencing; Microbial community structures; Quantitative real-time PCR (qPCR); Redundancy analysis; AMMONIA-OXIDIZING BACTERIA; NITRATE REDUCTION; CONSTRUCTED WETLAND; GENOME SEQUENCE; NITROGEN; ABUNDANCE; ARCHAEA; GENES; DENITRIFICATION; DIGESTION;
D O I：
10.1016/j.biortech.2015.03.072
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The microbial communities and abundance in anaerobic sludge from 4 industrial and 2 municipal wastewater treatment plants were investigated using 454 pyrosequencing technology in this study. A total of 5482-8692 high-quality reads of 16S rRNA V3-V5 regions were obtained. Taxonomic analysis using QIIME and RDP classifier found that Proteobacteria, Bacteroidetes, Chloroflexi and Firmicutes were the most abundant phyla in these samples. Furthermore, real-time PCR was used to validate the absolute abundance of these 16S rRNAs and some functional genes, including total bacteria, anammox bacteria, NOB (Nitrobacter, Nitrospira), AOA amoA, AOB amoA, nosZ, nirS, nirK, narG, napA, nrfA, mcrA and dsrA. Multivariate linear regression analysis indicated that AOA might be mixotrophic. Finally, redundancy analysis was used to reveal the relationships between operation parameters and microbial communities. Results showed that the coexistence of anammox, denitrification and DNRA could be useful for the simultaneous removal of nitrogen and organic matter. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：163 / 172
页数：10
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