Isolation and characterization of twelve polymorphic microsatellite loci in Polygonatum cyrtonema and cross-species amplification

被引：3

作者：

Cheng, Wen-juan ^{[1
,2
]}

Liu, Ting-ting ^{[1
,2
]}

Wu, Hai-long ^{[1
,2
]}

Zhou, Shou-biao ^{[1
,2
]}

Xuan, Shou-qin ^{[1
,2
]}

Zhu, Guo-ping ^{[1
,2
]}

机构：

[1] Anhui Normal Univ, Key Lab Mol Evolut & Biodivers, Coll Life Sci, Wuhu 241000, Peoples R China

[2] Anhui Normal Univ, Anhui Prov Key Lab Conservat & Exploitat Biol Res, Coll Life Sci, Wuhu 241000, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2010年 / 2卷

基金：

中国国家自然科学基金;

关键词：

Polygonatum cyrtonema; Microsatellite; Cross-amplification; Population genetics; EVOLUTION;

D O I：

10.1007/s12686-010-9218-1

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Twelve novel microsatellite loci were isolated in a perennial herb, Polygonatum cyrtonema. The polymorphism of each locus was analyzed by screening 23 individuals from a natural population. The number of alleles ranged from 2 to 11, and the observed and expected heterozygosities ranged from 0.273 to 0.727 and from 0.268 to 0.865, respectively. These microsatellite loci were cross-amplified for four congeneric species and nine loci can be amplified successfully in all four species. The results suggest that these microsatellite markers would contribute to the population genetic studies of P. cyrtonema and other related species.

引用

页码：105 / 107

页数：3

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