Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for Rapid Diagnosis of Sugarcane yellow leaf virus in Sugarcane

被引：5

作者：

Anandakumar, L. ^{[1
,2
]}

Bagyalakshmi, K. ^{[1
]}

Nithya, K. ^{[1
]}

Parameswari, B. ^{[3
]}

Viswanathan, R. ^{[1
]}

机构：

[1] Indian Council Agr Res, ICAR Sugarcane Breeding Inst, Div Crop Protect, Coimbatore 641007, Tamil Nadu, India

[2] Indian Inst Technol Kharagpur, Dept Agr & Food Engn, Kharagpur 721302, W Bengal, India

[3] ICAR Sugarcane Breeding Inst, Reg Ctr, Karnal 132001, Haryana, India

来源：

SUGAR TECH | 2018年 / 20卷 / 06期

关键词：

Sugarcane; Yellow leaf disease; Sugarcane yellow leaf virus; RT-LAMP; RT-PCR; qRT-PCR; QUANTIFICATION; POLYMERASE; DNA;

D O I：

10.1007/s12355-018-0606-2

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Loop-mediated isothermal amplification (LAMP) is a rapid, highly specific and reliable technique, which is widely incorporated in the field for diagnosis of pathogens at their early stage of infection. In present study, RT-LAMP assay was developed successfully to detect Sugarcane yellow leaf virus (SCYLV) causing yellow leaf disease in sugarcane and compared with conventional reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time polymerase chain reaction (qRT-PCR) to assess its sensitivity. RT-LAMP was able to detect the SCYLV up to a limit of 10pg (picogram), while RT-PCR assays were able to detect this virus up to 100pg only. The sensitivity of the RT-LAMP method was tenfold higher than that of conventional RT-PCR assay. This study revealed high sensitivity of RT-LAMP in detecting SCYLV in Indian sugarcane cultivars.

引用

页码：708 / 716

页数：9

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