Production of Large-Ring Cyclodextrins by Amylomaltases

被引：10

作者：

Krusong, Kuakarun ^{[1
]}

Ismail, Abbas ^{[1
]}

Wangpaiboon, Karan ^{[1
]}

Pongsawasdi, Piamsook ^{[2
]}

机构：

[1] Chulalongkorn Univ, Fac Sci, Dept Biochem, Struct & Computat Biol Res Unit, Phyathai Rd, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Fac Sci, Dept Biochem, Starch & Cyclodextrin Res Unit, Phyathai Rd, Bangkok 10330, Thailand

来源：

MOLECULES | 2022年 / 27卷 / 04期

关键词：

amylomaltase; cyclization; 4-alpha-glucanotransferases; large-ring cyclodextrin; starch; THERMUS-AQUATICUS; CYCLOAMYLOSE PRODUCTION; ESCHERICHIA-COLI; BIOCHEMICAL-CHARACTERIZATION; CORYNEBACTERIUM-GLUTAMICUM; ALPHA-GLUCANOTRANSFERASE; ENZYMATIC MODIFICATION; MALTOSE METABOLISM; CRYSTAL-STRUCTURE; COMPLEX-FORMATION;

D O I：

10.3390/molecules27041446

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amylomaltase is a well-known glucan transferase that can produce large ring cyclodextrins (LR-CDs) or so-called cycloamyloses via cyclization reaction. Amylomaltases have been found in several microorganisms and their optimum temperatures are generally around 60-70 & DEG;C for thermostable amylomaltases and 30-45 & DEG;C for the enzymes from mesophilic bacteria and plants. The optimum pHs for mesophilic amylomaltases are around pH 6.0-7.0, while the thermostable amylomaltases are generally active at more acidic conditions. Size of LR-CDs depends on the source of amylomaltases and the reaction conditions including pH, temperature, incubation time, and substrate. For example, in the case of amylomaltase from Corynebacterium glutamicum, LR-CD productions at alkaline pH or at a long incubation time favored products with a low degree of polymerization. In this review, we explore the synthesis of LR-CDs by amylomaltases, structural information of amylomaltases, as well as current applications of LR-CDs and amylomaltases.

引用

页数：15

共 90 条

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