Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells

被引:11
作者
Marta, Cadenas-Martin [1 ]
Adrian, Moratilla [1 ]
Jorge, Fernandez-Delgado [2 ]
Francisco, Arnalich-Montiel [3 ]
De Miguel, Maria P. [1 ]
机构
[1] La Paz Univ Hosp, Cell Engn Lab, IDiPAZ, Hlth Res Inst, Madrid 28046, Spain
[2] Santa Cristina Hosp & Centrocim, Dept Plast & Reconstruct Surg, Madrid 28009, Spain
[3] Ramon y Cajal Univ Hosp, Inst Ramon y Cajal Invest Sanitaria, Ophthalmol Dept, Madrid 28034, Spain
关键词
cell reprogramming; differentiation; adipose tissue-derived mesenchymal stem cells; cornea; corneal endothelium; CULTURE; TRANSPLANTATION; GENERATION; MEDIA;
D O I
10.3390/ijms222111982
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Corneal disease affects 12.5 million individuals worldwide, with 2 million new cases each year. The standard treatment consists of a corneal transplantation from a human donor; however, the worldwide demand significantly exceeds the available supply. Lamellar endothelial keratoplasty, the replacement of only the endothelial layer of the cornea, can partially solve the problem. Progressive efforts have succeeded in expanding hCECs; however, the ability to expand hCECs is still limited, and new sources of CECs are being sought. Crucial advances have been achieved by the directed differentiation of embryonic or induced pluripotent stem cells, but these cells have disadvantages, such as the use of oncogenes, and are still difficult to establish. We aimed to transfer such knowledge to obtain hCECs from adipose tissue-derived adult mesenchymal stem cells (ADSC) by modifying four previously published procedures. We present several protocols capable of the directed differentiation of human ADSCs to hCECs. In our hands, the protocol by Ali et al. was the best adapted to such differentiation in terms of efficiency, time, and financial cost; however, the protocol by Wagoner et al. was the best for CEC marker expression. Our results broaden the type of cells of autologous extraocular origin that could be employed in the clinical setting for corneal endothelial deficiency.
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页数:13
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