In vivo imaging of cellular structures in Caenorhabditis elegans by combined TPEF, SHG and THG microscopy

被引：31

作者：

Gualda, E. J. ^{[1
]}

Filippidis, G. ^{[1
]}

Voglis, G. ^{[2
]}

Mari, M. ^{[1
,3
]}

Fotakis, C. ^{[1
,3
]}

Tavernarakis, N. ^{[2
]}

机构：

[1] Fdn Res & Technol Hellas, Inst Elect Struct & Laser, Iraklion 71110, Crete, Greece

[2] Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion, Crete, Greece

[3] Univ Crete, Dept Phys, Crete, NE USA

来源：

JOURNAL OF MICROSCOPY | 2008年 / 229卷 / 01期

关键词：

Caenorhabditis elegans; imaging; microscopy; SHG; THG; TPEF;

D O I：

10.1111/j.1365-2818.2007.01876.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

In this study, we use combined two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG) and third-harmonic generation (THG) measurements to image cellular structures of the nematode Caenorhabditis elegans, in vivo. To our knowledge, this is the first time that a THG modality is employed to image live C. elegans specimens. Femtosecond laser pulses (1028 nm) were utilized for excitation. Detailed and specific structural and anatomical features can be visualized, by recording THG signals. Thus, the combination of three image-contrast modes (TPEF-SHG-THG) in a single instrument has the potential to provide unique and complementary information about the structure and function of tissues and individual cells of live biological specimens.

引用

页码：141 / 150

页数：10

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