Properties of naphtho[2,1-b]pyrans covalently linked to oligonucleotides

被引：3

作者：

Mazzei, M ^{[1
]}

Sottofattori, E ^{[1
]}

Ibrahim, M ^{[1
]}

Balbi, A ^{[1
]}

机构：

[1] Dept Pharmaceut Sci, I-16132 Genoa, Italy

来源：

NUCLEOSIDES & NUCLEOTIDES | 1998年 / 17卷 / 9-11期

关键词：

D O I：

10.1080/07328319808004727

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two naphtho[2,1-b]pyrans (benzocoumarin 1 and benzochromone 2) were covalently linked to a 7-mer ODN. Purification and hydrophobicity studies of the resulting conjugates 1* and 2* were performed by HPLC. Benzochromone conjugate 2* was more hydrophobic than benzocoumarin conjugate 1*. Preliminary data on thermal stability and interaction with HIV reverse transcriptase showed that 1* is slightly more efficient than 2*. This is probably due to the steric hindrance of the 3-diethylamino group in 2.

引用

收藏

页码：1885 / 1894

页数：10

相关论文

共 15 条

[1] Inhibition of DNA polymerase alpha expression and cell growth, a possible triple helix mechanism [J].

Alama, A ;

Barbieri, F ;

Cagnoli, M ;

Mazzei, M ;

Grandi, T ;

Nicolin, A .

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 1996, 6 (02) :95-101

[2] SYNTHESIS AND COMPLEMENTARY COMPLEX-FORMATION PROPERTIES OF OLIGONUCLEOTIDES COVALENTLY-LINKED TO NEW STABILIZING AGENTS [J].

BALBI, A ;

SOTTOFATTORI, E ;

GRANDI, T ;

MAZZEI, M ;

ABRAMOVA, TV ;

LOKHOV, SG ;

LEBEDEV, AV .

TETRAHEDRON, 1994, 50 (13) :4009-4018

[3]

BALBI A, 1997, IN PRESS BIOORGANIC

[4]

BICHENKOVA EV, 1992, BIOORG KHIM+, V18, P398

[5]

ERMILI A, 1971, GAZZ CHIM ITAL, V101, P269

[6]

HELENE C, 1991, ANTI-CANCER DRUG DES, V6, P569

[7] THERAPEUTIC IMPLICATIONS OF ANTISENSE OLIGONUCLEOTIDES [J].

LATTUADA, D ;

MAZZEI, M ;

MEAZZA, R ;

NICOLIN, A .

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, 1992, 21 (04) :296-299

[8] MODE OF ACTION OF 2-(DIETHYLAMINO)-7-ETHOXYCHROMONE ON HUMAN PLATELETS [J].

LEONCINI, G ;

MARESCA, M ;

COLAO, C ;

BUZZI, E ;

MAZZEI, M .

CELL BIOCHEMISTRY AND FUNCTION, 1991, 9 (02) :79-85

[9] NEW POLYCYCLIC PYRAN RING-SYSTEMS [J].

MAZZEI, M ;

ROMA, G ;

ERMILI, A .

JOURNAL OF HETEROCYCLIC CHEMISTRY, 1978, 15 (04) :605-608

[10] SYNTHESIS AND ANTIPLATELET ACTIVITY OF 2-(DIETHYLAMINO)-7-ETHOXYCHROMONE AND RELATED-COMPOUNDS [J].

MAZZEI, M ;

SOTTOFATTORI, E ;

DIBRACCIO, M ;

BALBI, A ;

LEONCINI, G ;

BUZZI, E ;

MARESCA, M .

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 1990, 25 (07) :617-622