Northern analysis of type II iodothyronine deiodinase mRNA in rat Harderian gland

被引：7

作者：

Araki, O ^{[1
]}

Murakami, M ^{[1
]}

Kamiya, Y ^{[1
]}

Hosoi, Y ^{[1
]}

Ogiwara, T ^{[1
]}

Mizuma, H ^{[1
]}

Iriuchijima, T ^{[1
]}

Mori, M ^{[1
]}

机构：

[1] Gunma Univ, Sch Med, Dept Internal Med 1, Maebashi, Gumma 3718511, Japan

来源：

LIFE SCIENCES | 1998年 / 63卷 / 21期

关键词：

thyroid hormone; adrenergic receptor; hypothyroidism; isoproterenol;

D O I：

10.1016/S0024-3205(98)00471-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

It has been known that type II iodothyronine deiodinase activity is present in rat Harderian gland and the activity is significantly increased by isoproterenol administration. We have performed Northern analyses to study whether the transcript for type II iodothyronine deiodinase is expressed in rat Harderian gland and whether the isoproterenol stimulation of type II iodothyronine deiodinase activity in rat Harderian gland is due to the change in its mRNA lever. Northern analyses have demonstrated that type II iodothyronine deiodinase mRNA, approximately 7.5 kb in size, is expressed in rat Harderian gland, and the mRNA levels as well as the deiodinase activities are greater in hypothyroid rats than those in euthyroid rats. Type II iodothyronine deiodinase mRNA levels and the deiodinase activities in Harderian gland were increased by isoproterenol administration, and the increase in the mRNA levels preceded that in the deiodinase activities. These results indicate that 7.5 kb transcript for type II iodothyronine deiodinase is expressed in rat Harderian gland and beta-adrenergic stimulation of type II iodothyronine deiodinase activity is due at least in part to the increase in its mRNA level.

引用

页码：1843 / 1848

页数：6

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