Isoflavonoids and peptides from meju, long-term fermented soybeans, increase insulin sensitivity and exert insulinotropic effects in vitro

被引:80
作者
Kwon, Dae Young [2 ]
Hong, Sang Mee [1 ]
Ahn, Il Sung [1 ]
Kim, Min Jung [2 ]
Yang, Hye Jeong [2 ]
Park, Sunmin [1 ]
机构
[1] Hoseo Univ, Dept Food & Nutr, Obes Diabet Ctr, Asan, Chungnam Do, South Korea
[2] Korean Food Res Inst, Food Funct Res Div, Songnam, South Korea
关键词
Fermented soybeans; Proliferator-activated receptor-gamma; Insulin-sensitizing action; Insulinotropic action; Glucagon-like peptide-1 secretion; GLUCAGON-LIKE PEPTIDE-1; PANCREATIC BETA-CELLS; IRS2 KNOCKOUT MICE; DIABETES-MELLITUS; SECRETION; GAMMA; RATS; CHUNGKOOKJANG; DEFICIENCY; EXENDIN-4;
D O I
10.1016/j.nut.2010.02.004
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Objective: Although soybeans have been shown to alleviate metabolic syndromes, fermented soybeans may have even greater effects. We investigated the antidiabetic effects of meju, a soy food that is fermented up to 2 mo, and the mechanism by which it exerts its effects. Methods: Meju was prepared by a traditional fermentation process: soybeans were fermented outdoors for 20 or 60 d. Methanol (M-60) and water (W-60) extracts from meju that had fermented for 60 d contained mostly isoflavonoid aglycones and small peptides, respectively, as opposed to mostly glycosylated isoflavonoids and proteins in the original soybeans. Results: Daidzein, M-60, and W-60 had better insulin-sensitizing actions by activating peroxisome proliferator-activated receptor-gamma in 3T3-L1 adipocytes than did unfermented soybeans. In addition, Min6 insulinoma cells treated with genistein, M-60, and W-60 had greater glucose-stimulated insulin secretion capacity and greater beta-cell viability than those treated with unfermented soybeans. This improvement was associated with insulin/insulin-like growth factor-1 signaling that was activated by the tyrosine phosphorylation of insulin receptor substrate-2 and serine phosphorylation of Akt, and this in turn increased pancreatic and duodenal homeobox-1 expression. Furthermore, genistein, daidzein, and M-60 stimulated glucagon-like peptide-1 secretion in enteroendocrine NCI-H716 cells, which generated insulinotropic actions. Conclusion: The compositional changes in isoflavonoids and peptides that occurred during a longer fermentation period, without the use of salt, enhanced the antidiabetic effect of soybeans. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:244 / 252
页数:9
相关论文
共 33 条
[1]   Thiazolidinediones produce a conformational change in peroxisomal proliferator-activated receptor-gamma: Binding and activation correlate with antidiabetic actions in db/db mice [J].
Berger, J ;
Bailey, P ;
Biswas, C ;
Cullinan, CA ;
Doebber, TW ;
Hayes, NS ;
Saperstein, R ;
Smith, RG ;
Leibowitz, MD .
ENDOCRINOLOGY, 1996, 137 (10) :4189-4195
[2]   Beneficial role of dietary phytoestrogens in obesity and diabetes [J].
Bhathena, SJ ;
Velasquez, MT .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 2002, 76 (06) :1191-1201
[3]   The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway [J].
Choi, SB ;
Wha, JD ;
Park, S .
LIFE SCIENCES, 2004, 75 (22) :2653-2664
[4]   Enhancing incretin action for the treatment of type 2 diabetes [J].
Drucker, DJ .
DIABETES CARE, 2003, 26 (10) :2929-2940
[5]   Pioglitazone induces in vivo adipocyte differentiation in the obese Zucker fa/fa rat [J].
Hallakou, S ;
Doare, L ;
Foufelle, F ;
Kergoat, M ;
GuerreMillo, M ;
Berthault, MF ;
Dugail, I ;
Morin, J ;
Auwerx, J ;
Ferre, P .
DIABETES, 1997, 46 (09) :1393-1399
[6]   Upregulation of insulin receptor substrate-2 in pancreatic β cells prevents diabetes [J].
Hennige, AM ;
Burks, DJ ;
Ozcan, U ;
Kulkarni, RN ;
Ye, J ;
Park, SM ;
Schubert, M ;
Fisher, TL ;
Dow, MA ;
Leshan, R ;
Zakaria, M ;
Mossa-Basha, M ;
White, MF .
JOURNAL OF CLINICAL INVESTIGATION, 2003, 112 (10) :1521-1532
[7]   Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120 [J].
Hirasawa, A ;
Tsumaya, K ;
Awaji, T ;
Katsuma, S ;
Adachi, T ;
Yamada, M ;
Sugimoto, Y ;
Miyazaki, S ;
Tsujimoto, G .
NATURE MEDICINE, 2005, 11 (01) :90-94
[8]   Glucagon-like peptide-1 synthetic analogs: New therapeutic agents for use in the treatment of diabetes mellitus [J].
Holz, GG ;
Chepurny, OG .
CURRENT MEDICINAL CHEMISTRY, 2003, 10 (22) :2471-2483
[9]  
Jang CH, 2008, FOOD SCI BIOTECHNOL, V17, P442
[10]   Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1 [J].
Jang, Hyeung-Jin ;
Kokrashvili, Zaza ;
Theodorakis, Michael J. ;
Carlson, Olga D. ;
Kim, Byung-Joon ;
Zhou, Jie ;
Kim, Hyeon Ho ;
Xu, Xiangru ;
Chan, Sic L. ;
Juhaszova, Magdalena ;
Bernier, Michel ;
Mosinger, Bedrich ;
Margolskee, Robert F. ;
Egan, Josephine M. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (38) :15069-15074