The Utility of Poly(γ-glutamic acid) Nanoparticles as Antigen Delivery Carriers in Dendritic Cell-Based Cancer Immunotherapy

被引：16

作者：

Matsuo, Keisuke ^{[1
]}

Ishii, Yumiko ^{[1
]}

Matsuo, Kazuhiko ^{[1
]}

Yoshinaga, Tomoyo ^{[1
]}

Akashi, Mitsuru ^{[2
,3
]}

Mukai, Yohei ^{[1
]}

Yoshioka, Yasuo ^{[1
,2
]}

Okada, Naoki ^{[1
]}

Nakagawa, Shinsaku ^{[1
,2
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Biotechnol & Therapeut, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Ctr Adv Med Engn & Informat, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Grad Sch Engn, Dept Appl Chem, Suita, Osaka 5650871, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2010年 / 33卷 / 12期

关键词：

poly(gamma glutamic acid) nanoparticle; antigen delivery carrier; dendritic cell; AMPHIPHILIC GAMMA-PGA; CLASS-I PRESENTATION; MHC CLASS-I; TUMOR-VACCINE; T-CELLS; MATURATION; IMMUNITY; MOLECULES; RESPONSES; CD80;

D O I：

10.1248/bpb.33.2003

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Cytotoxic T-lymphocytes (CTLs) specific for tumor-associated antigens (TAAs) act in the immune surveillance system as major effector cells to eliminate malignant cells Immunization with TAA-loaded dendritic cells (DCs) has great potential for treating cancer, because DCs are potent antigen-presenting cells capable of inducing antigen-specific CTLs by the primary activation of naive T-lymphocytes The establishment of a non-cyto-toxic and efficient antigen delivery method is required to Improve the efficacy of DC-based cancer immunotherapy We developed biodegradable poly(gamma-glutamic acid) nanoparticles (gamma-PGA NPs) that can efficiently entrap various proteins as antigen delivery carriers gamma-PGA NPs efficiently delivered entrapped antigenic proteins into DCs without cytotoxicity and presented antigens to DCs via major histocompatibility complex class I and II molecules Immunization with TAA-loaded DCs using gamma-PGA NPs inhibited tumor growth by inducing TAA-specific CTLs These findings indicate that gamma-PGA NPs can function as useful antigen delivery carriers in DC-based cancer Immunotherapy

引用

页码：2003 / 2007

页数：5

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