A systematic screen for protein-lipid interactions in Saccharomyces cerevisiae

被引：129

作者：

Gallego, Oriol ^{[1
]}

Betts, Matthew J. ^{[1
]}

Gvozdenovic-Jeremic, Jelena ^{[1
]}

Maeda, Kenji ^{[1
]}

Matetzki, Christian ^{[1
]}

Aguilar-Gurrieri, Carmen ^{[1
]}

Beltran-Alvarez, Pedro ^{[1
]}

Bonn, Stefan ^{[1
]}

Fernandez-Tornero, Carlos ^{[1
]}

Jensen, Lars Juhl ^{[1
]}

Kuhn, Michael ^{[1
]}

Trott, Jamie ^{[1
]}

Rybin, Vladimir ^{[2
]}

Mueller, Christoph W. ^{[1
]}

Bork, Peer ^{[1
]}

Kaksonen, Marko ^{[3
]}

Russell, Robert B. ^{[1
]}

Gavin, Anne-Claude ^{[1
]}

机构：

[1] European Mol Biol Lab, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

[2] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

[3] European Mol Biol Lab, Prot Express & Purificat Core Facil, Heidelberg, Germany

来源：

MOLECULAR SYSTEMS BIOLOGY | 2010年 / 6卷

关键词：

interactome; lipid-array; network; pleckstrin homology domains; sphingolipids; PLECKSTRIN-HOMOLOGY-DOMAIN; GLOBAL ANALYSIS; PH DOMAIN; BINDING; YEAST; PHOSPHOINOSITIDES; IDENTIFICATION; KINASES; GENOMES; SLM1;

D O I：

10.1038/msb.2010.87

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein-metabolite networks are central to biological systems, but are incompletely understood. Here, we report a screen to catalog protein-lipid interactions in yeast. We used arrays of 56 metabolites to measure lipid-binding fingerprints of 172 proteins, including 91 with predicted lipid-binding domains. We identified 530 protein-lipid associations, the majority of which are novel. To show the data set's biological value, we studied further several novel interactions with sphingolipids, a class of conserved bioactive lipids with an elusive mode of action. Integration of live-cell imaging suggests new cellular targets for these molecules, including several with pleckstrin homology (PH) domains. Validated interactions with Slm1, a regulator of actin polarization, show that PH domains can have unexpected lipid-binding specificities and can act as coincidence sensors for both phosphatidylinositol phosphates and phosphorylated sphingolipids. Molecular Systems Biology 6: 430; published online 30 November 2010; doi:10.1038/msb.2010.87

引用

页数：15

共 50 条

[11] On protein-lipid membrane interactions
Ramsden, JJ
COLLOIDS AND SURFACES B-BIOINTERFACES, 1999, 14 (1-4) : 77 - 81
[12] VEGETABLE PROTEIN-LIPID INTERACTIONS
KAMAT, VB
GRAHAM, GE
DAVIS, MAF
CEREAL CHEMISTRY, 1978, 55 (03) : 295 - 307
[13] PROTEIN-LIPID INTERACTIONS IN BIOMEMBRANES
CHAPMAN, D
GOMEZ, JC
GONI, FM
HOFFMANN, W
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1978, 359 (09): : 1069 - 1070
[14] PROTEIN-LIPID INTERACTIONS IN MYELIN
BRAUN, PE
RADIN, NS
FEDERATION PROCEEDINGS, 1969, 28 (02) : 404 - &
[15] Biophysics of protein-lipid interactions
Bender, Paula A.
Jayaraman, Vasanthi
BIOPHYSICAL JOURNAL, 2024, 123 (14) : 1912 - 1914
[16] Protein-lipid interactions at interfaces
Fillery-Travis, A
Mills, ENC
Wilde, P
GRASAS Y ACEITES, 2000, 51 (1-2) : 50 - 55
[17] An in vivo screen reveals protein-lipid interactions crucial for gating a mechanosensitive channel
Iscla, Irene
Wray, Robin
Blount, Paul
FASEB JOURNAL, 2011, 25 (02): : 694 - 702
[18] Systematic analysis of protein domain interactions in Saccharomyces cerevisiae.
Uetz, P
Vollert, CS
Dambmann, C
Heucke, S
Rajagopala, SV
Schüler, T
YEAST, 2003, 20 : S272 - S272
[19] Probing membrane protein-lipid interactions
Agasid, Mark T.
Robinson, Carol, V
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2021, 69 : 78 - 85
[20] Specificity of Intramembrane Protein-Lipid Interactions
Contreras, Francesc-Xabier
Ernst, Andreas Max
Wieland, Felix
Bruegger, Britta
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2011, 3 (06): : 1 - 18

← 1 2 3 4 5 →