Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.]

被引:1
|
作者
Ajay, B. C. [1 ]
Gowda, M. Byre [1 ]
Prasad, P. S. [1 ]
Veerakumar, G. N. [1 ]
Babu, El. Prashanth [1 ]
Gnanesh, B. N. [1 ]
Venkatesh, S. C. [1 ]
Ganapathy, K. N. [1 ]
Fiyaz, R. Abdul [1 ]
Ramya, K. T. [1 ]
机构
[1] Uniers Agr Sci, GKVK, ZARS, AICRP Pigeonpea, Bangalore, Karnataka, India
关键词
AFLP; BSA; Fusarium wilt disease; Linkage; Pigeonpea; GENETIC-LINKAGE MAP; RESISTANCE GENES; MICROSATELLITE; RAPD;
D O I
10.5958/0976-0571.2015.00021.1
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
An experiment was conducted to identify markers linked to Fusarium wilt disease resistance, Parents namely TTB 7 and ICP 8863 were screened using 151 SSRs markers and 16 AFLP primer combinations. Parental screening revealed five SSR primers and 12 AFLP primer combinations polymorphic between parents. Bulk segregant analysis identified five AFLP primer combinations generating seven markers polymorphic between resistant and susceptible bulks while, none of the SSR markers were polymorphic. This indicates that, these markers are putatively linked to wilt disease. Screening of F-2 segregating population of cross TTB 7 x ICP 8863 with these putatively linked markers revealed four markers (E-AAT/M-CTG(850), E-TCG/M-CTT650, E-TCG/M-CTT730 and E-TCG/M-CTT230) which segregated in 3:1 mendelian pattern. Simple linear regression performed on these four markers had identified two markers namely E-TCG/M-CTT650 and E-TCG/M-CTA(730) linked to disease.
引用
收藏
页码:126 / 130
页数:5
相关论文
共 50 条
  • [1] Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.)
    H. Kotresh
    B. Fakrudin
    S. M. Punnuri
    B. K. Rajkumar
    M. Thudi
    H. Paramesh
    H. Lohithswa
    M. S. Kuruvinashetti
    Euphytica, 2006, 149 : 113 - 120
  • [2] Genetic Analysis of Pigeonpea (Cajanus cajan L. Millsp.) Genotypes for Fusarium wilt using Microsatellite Markers
    Prajapati, Vijay
    Sasidharan, N.
    Soni, Nishit
    Patel, Ankita
    Prajapat, Pravin
    JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2016, 10 (02): : 1559 - 1563
  • [3] Identification of sources of resistance to Fusarium wilt and sterility mosaic diseases in pigeonpea [Cajanus cajan (L.) Millsp.]
    Kashyap, G. S. Sinchana
    Lohithaswa, H. C.
    Banakara, Santhoshkumari
    Sowmya, M. S.
    Padmaja, A. S.
    Keshavareddy, G.
    Mallikarjuna, M. G.
    Gangashetty, Prakash
    INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 2023, 83 (02) : 278 - 281
  • [4] New microsatellite markers for pigeonpea (cajanus cajan (L.) millsp.)
    Odeny D.
    Jayashree B.J.
    Gebhardt C.
    Crouch J.
    BMC Research Notes, 2 (1)
  • [5] Inheritance studies on Fusarium wilt resistance in long duration pigeonpea [Cajanus cajan (L.) Millsp.]
    Singh, Anil Kumar
    Singh, Dhirendra Kumar
    Kumar, Rajeev
    Singh, Mahendra Narain
    Rai, Ved Prakash
    LEGUME RESEARCH, 2018, 41 (06) : 934 - 937
  • [6] VARIANT SPECIFIC STUDIES ON INHERITANCE FOR RESISTANCE TO FUSARIUM WILT IN PIGEONPEA[CAJANUS CAJAN (L.) MILLSP.]
    Patil, Prakash
    Singh, I. P.
    Dhar, Vishwa
    Chaudhary, R. G.
    Datta, S.
    Chaturvedi, S. K.
    Nadarajan, N.
    LEGUME RESEARCH, 2013, 36 (06) : 576 - 581
  • [7] AFLP Fingerprinting in Pigeonpea (Cajanus cajan (L.) Millsp.) and its Wild Relatives
    S. K. Panguluri
    K. Janaiah
    J. N. Govil
    P. A. Kumar
    P. C. Sharma
    Genetic Resources and Crop Evolution, 2006, 53 : 523 - 531
  • [8] AFLP fingerprinting in pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives
    Panguluri, SK
    Janaiah, K
    Govil, JN
    Kumar, PA
    Sharma, PC
    GENETIC RESOURCES AND CROP EVOLUTION, 2006, 53 (03) : 523 - 531
  • [9] Genetic analysis and molecular resistance to race 2 of Fusarium wilt in pigeonpea [Cajanus cajan (L.) Millsp.]
    Patil, Prakash G.
    Bohra, Abhishek
    Dubey, Jyotirmay
    Saabale, Parasappa R.
    Mishra, Raj K.
    Pandey, Gaurav
    Das, Alok
    Rathore, Meenal
    Singh, Farindra
    Singh, Narendra Pratap
    CROP PROTECTION, 2017, 100 : 117 - 123
  • [10] The potential of pigeonpea (Cajanus cajan (L.) Millsp.) in Africa
    Odeny, Damaris Achieng
    NATURAL RESOURCES FORUM, 2007, 31 (04) : 297 - 305