Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

被引：1471

作者：

Christian, Michelle ^{[1
,2
]}

Cermak, Tomas ^{[1
,2
]}

Doyle, Erin L. ^{[3
]}

Schmidt, Clarice ^{[3
]}

Zhang, Feng ^{[1
,2
]}

Hummel, Aaron ^{[3
]}

Bogdanove, Adam J. ^{[3
]}

Voytas, Daniel F. ^{[1
,2
]}

机构：

[1] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Ctr Genome Engn, Minneapolis, MN 55455 USA

[3] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA

来源：

GENETICS | 2010年 / 186卷 / 02期

基金：

美国国家科学基金会;

关键词：

ZINC-FINGER NUCLEASES; HUMAN-CELLS; GENE; RECOGNITION;

D O I：

10.1534/genetics.110.120717

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.

引用

收藏

页码：757 / U476

页数：13

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