The assessment of rat ureteral pressure generation in vitro: regional heterogeneity and influence of distending pressure

被引:2
作者
Rasidovic, D. [1 ]
Kearney, D. [1 ]
Boyle, Kate-Marie [1 ]
Bund, S. J. [1 ]
机构
[1] Univ Coll Dublin, Sch Med & Med Sci, Hlth Sci Ctr, Dublin 4, Ireland
关键词
acetylcholine; contraction; pressure; smooth muscle; ureter; ureteral lumenal pressure; nifedipine extracellular Ca2+; ureteral contractile responses; UPPER URINARY-TRACT; MESENTERIC SMALL ARTERIES; CONTRACTILE PROPERTIES; MUSCLE; DEPOLARIZATION; SENSITIVITY; INNERVATION; OBSTRUCTION; MOTILITY; AGONISTS;
D O I
10.1556/APhysiol.97.2010.3.7
中图分类号
学科分类号
摘要
Contraction of ureteral smooth muscle drives the urine bolus to the urinary bladder for storage prior to micturition. This study describes a novel approach to the measurement of ureteral pressure generation in vitro and the influence of distending pressure on acetylcholine-stimulated ureteral lumenal pressure generation. Isolated segments of ureters obtained from Wistar rats were pressurised in a blind-ended sac arrangement and contractile responses were recorded as phasic oscillations in ureteral luminal pressure. Distal segments generated greater luminal pressures than proximal segments (p<0.001) in response to acetylcholine. Increasing baseline distending pressures in the range 2-10 mmHg in proximal segments was associated with greater frequency of contraction (p<0.001) and decreased magnitude of contraction (p<0.001) when expressed as % maximum response. Nifedipine (10(-5) M) or removal of extracellular Ca2+ abolished the contractions. Isometric contractile responses of ureteral ring preparations were not significantly influenced by pretensions equivalent to distending pressures in the range 2-10 mmHg. This is the first study to fully establish the influence of baseline ureteral distending pressure upon ureteral luminal pressure generation in vitro and demonstrates regional heterogeneity of ureteral contractile responses. It is suggested that this experimental approach may be a useful methodology for the investigation of ureteral function during urinary outflow obstruction.
引用
收藏
页码:307 / 315
页数:9
相关论文
共 21 条
[1]   The urodynamic relationship of renal pelvic and bladder pressures, and urinary flow rate in rats with congenital vesicoureteral reflux [J].
Angell, SK ;
Pruthi, RS ;
Shortliffe, LD .
JOURNAL OF UROLOGY, 1998, 160 (01) :150-156
[2]  
ARAGONA F, 1988, International Urology and Nephrology, V20, P239, DOI 10.1007/BF02549510
[3]   DIFFERENCES IN SENSITIVITY OF RAT MESENTERIC SMALL ARTERIES TO AGONISTS WHEN STUDIED AS RING PREPARATIONS OR AS CANNULATED PREPARATIONS [J].
BUUS, NH ;
VANBAVEL, E ;
MULVANY, MJ .
BRITISH JOURNAL OF PHARMACOLOGY, 1994, 112 (02) :579-587
[4]   Multiple comparisons: philosophies and illustrations [J].
Curran-Everett, D .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2000, 279 (01) :R1-R8
[5]   ENHANCED RESISTANCE ARTERY SENSITIVITY TO AGONISTS UNDER ISOBARIC COMPARED WITH ISOMETRIC CONDITIONS [J].
DUNN, WR ;
WELLMAN, GC ;
BEVAN, JA .
AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (01) :H147-H155
[6]   VASCULAR MUSCLE-CELL DEPOLARIZATION AND ACTIVATION IN RENAL-ARTERIES ON ELEVATION OF TRANSMURAL PRESSURE [J].
HARDER, DR ;
GILBERT, R ;
LOMBARD, JH .
AMERICAN JOURNAL OF PHYSIOLOGY, 1987, 253 (04) :F778-F781
[7]   Contractile properties of ureters from rats with infravesical urinary outlet obstruction [J].
Hjortswang, H ;
Malmqvist, U ;
Uvelius, B ;
Arner, A .
UROLOGICAL RESEARCH, 1998, 26 (05) :337-342
[8]  
HOYES AD, 1976, INVEST UROL, V14, P38
[9]   Effects of artificial calculosis on rat ureter motility: Peripheral contribution to the pain of ureteric colic [J].
Laird, JMA ;
Roza, C ;
Cervero, F .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1997, 272 (05) :R1409-R1416
[10]   Electrical basis of peristalsis in the mammalian upper urinary tract [J].
Lang, RJ ;
Exintaris, B ;
Teele, ME ;
Harvey, J ;
Klemm, MF .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1998, 25 (05) :310-321