Capillary zone electrophoresis separation of basic proteins using arginine and lysine as buffer additives

The separation of basic proteins by capillary electrophoresis was plagued by the adsorption of the basic proteins onto the capillary inner surface, A method is presented to solve this problem by using arginine and lysine as buffer additives, It was found that in the neutral or slightly alkaline solution, arginine and lysine can be adsorbed onto the inner surface of capillary to reduce the electroosmotic flow and the adsorption of the proteins efficiently, which results in improvement of resolution efficiency and reproducibility of the migration time and peak height of basic proteins, The experimental results show that arginine and lysine are the efficient buffer additives used in separation of basic proteins.