Role of Vasoactive Intestinal Peptide in Hyperoxia-Induced Injury of Primary Type II Alveolar Epithelial Cells

被引：4

作者：

Ao, Xiaoxiao ^{[1
]}

Fang, Fang ^{[1
]}

Xu, Feng ^{[1
]}

机构：

[1] Chongqing Med Univ, Childrens Hosp, Chongqing 400014, Peoples R China

来源：

INDIAN JOURNAL OF PEDIATRICS | 2011年 / 78卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Vasoactive intestinal peptide; Type II alveolar epithelial cell; Hyperoxia; Oxidative stress; CYCLASE-ACTIVATING POLYPEPTIDE; LUNG INJURY; DEATH; VIP; PATHWAYS; INFLAMMATION; MACROPHAGES; RECEPTORS; APOPTOSIS; TISSUE;

D O I：

10.1007/s12098-010-0248-1

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

We investigated the effect of VIP on primary type II alveolar epithelial cells (AECIIs) upon the exposure of hyperoxia. AECIIs were isolated and purified from premature rats and exposed to air (21% oxygen), hyperoxia(95% oxygen), VIP+air and VIP+hyperoxia, respectively. The proliferation and apoptosis of AECIIs were detected by MTT cell proliferation assay, flow cytometry and western blot. The production of intracellular reactive oxygen species (ROS) was determined by 2 ', 7'-dichloro-dihydrotestosterone fluorescein diacetate (DCFH-DA) molecular probe and the total antioxidant capacity (TAOC) by ultraviolate spectro-photometer. Cell proliferation significantly increased and apoptosis decreased upon the treatment with VIP. In addition, the level of ROS in the hyperoxia+VIP group was significantly lower than in the hyperoxia group, in contrast, TAOC was higher in the hyperoxia+VIP group than that in the hyperoxia group. VIP exerts a protective role in the hyperoxia-induced oxidative stress damage in AECIIs, which probably attributed to its anti-oxidant and anti-apoptosis property.

引用

页码：535 / 539

页数：5

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