Tagging cells with quantum dots by electroporation

被引：0
|
作者：
Yang, R
Ji, W
Yan, YX
Shi, YY
Fei, Q
Mu, Y
Jin, QH [1 ]
机构：
[1] Jilin Univ, Jilin Prov Res Ctr Engn Technol Spectral Instumen, Inst Miniature Instrumentat, Coll Chem, Changchun 130023, Peoples R China
[2] Jilin Univ, Key Lab Mol Enzymol Engn, Minist Educ, Changchun 130023, Peoples R China
[3] Jilin Univ, Coll Basic Med, Changchun 130021, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2005年 / 26卷 / 06期
关键词：
quantum dot; cell tagging; electroporation;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An electroporation method was proposed to make QDs pass through the membranes of K562 leukaemia cells. This method is operated simply and facilitates the tagging experiment greatly. The results show that when the electroporation voltage is 200 V and the lasting time is 1 ms, a high tagging rate is reached. A lot of cells can be tagged at the same time and the livability of the cells is 60%. The whole process can be completed in 20 min. This method brings forward some more extrusive advantages than cell endocytosis and microinjection method, and there should have a very good foreground for further applications.
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收藏
页码：1043 / 1045
页数：3
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