Intrascleral Transplantation of a Collagen Sheet with Cultured Brain-Derived Neurotrophic Factor Expressing Cells Partially Rescues the Retina from Damage due to Acute High Intraocular Pressure

被引：6

作者：

Abe, Toshiaki ^{[1
]}

Tokita-Ishikawa, Yumi ^{[1
]}

Onami, Hideyuki ^{[1
]}

Katsukura, Yuki ^{[1
]}

Kaji, Hirokazu ^{[2
]}

Nishizawa, Matsuhiko ^{[2
]}

Nagai, Nobuhiro ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Div Clin Cell Therapy, Ctr Adv Med Res & Dev ART, Sendai, Miyagi 980, Japan

[2] Tohoku Univ, Grad Sch Engn, Dept Bioengn & Robot, Sendai, Miyagi 980, Japan

来源：

RETINAL DEGENERATIVE DISEASES: MECHANISMS AND EXPERIMENTAL THERAPY | 2014年 / 801卷

关键词：

Brain-derived neurotrophic factor; Collagen sheet; Transplantation; Sclera; Acute high ocular pressure; TRANSSCLERAL DRUG-DELIVERY; ISCHEMIA; BDNF;

D O I：

10.1007/978-1-4614-3209-8_105

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We constructed brain-derived neurotrophic factor (BDNF) expressing rat retinal pigment epithelial (RPE) cells by stable transfection of BDNF cDNA, and the RPE cells were cultured on a cross-linked collagen sheet (Coll-RPE-BDNF). BDNF expression of the Coll-RPE-BDNF was confirmed by western blot, and the Coll-RPE-BDNF was transplanted into the rabbit sclera. In vivo BDNF expression was confirmed by His expression that was linked to the expressing BDNF. The effect of the released BDNF was examined in a rabbit acute high intraocular pressure system by electroretinogram and histological examination. Statistically significant preservation of ERG b wave amplitude was observed in the rabbits treated by Coll-RPE-BDNF when compared to that of no treatment. Statistically significant preservation of the thickness of the inner nuclear layer at the transplanted area was observed in the rabbits treated by Coll-RPE-BDNF compared to that of no treatment. Intra-scleral Coll-RPE-BDNF transplantation may partially rescue retinal cells from acute high intraocular pressure.

引用

页码：837 / 843

页数：7

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