Antiviral activity and mechanism of ESC-1GN from skin secretion of Hylarana guentheri against influenza A virus

被引：7
作者：
Yang, Jie [1 ,2 ]
Zhang, Bei [1 ,2 ]
Huang, Yingna [1 ,2 ]
Liu, Teng [1 ,2 ]
Zeng, Baishuang [1 ,2 ]
Chai, Jinwei [1 ,2 ]
Wu, Jiena [1 ,2 ]
Xu, Xueqing [1 ,2 ]
机构：
[1] Southern Med Univ, Sch Pharmaceut Sci, Guangdong Prov Key Lab New Drug Screening, Shatai Nan Rd 1023-1063, Guangzhou 510515, Guangdong, Peoples R China
[2] Southern Med Univ, Guangzhou Key Lab Drug Res Emerging Virus Prevent, Sch Pharmaceut Sci, Shatai Nan Rd 1023-1063, Guangzhou 510515, Guangdong, Peoples R China
来源：
JOURNAL OF BIOCHEMISTRY | 2021年 / 169卷 / 06期
基金：
中国国家自然科学基金;
关键词：
amphibian; antimicrobial peptide; ESC-1GN; haemagglutinin; influenza A virus; H5N1 AVIAN INFLUENZA; ANTIMICROBIAL PEPTIDES; INFECTION; HEMAGGLUTININ; FROG; RESISTANCE; STABILITY;
D O I：
10.1093/jb/mvab019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Development of new and effective antiinfluenza drugs is critical for prophylaxis and treatment of influenza A virus (IAV) infection. A wide range of amphibian skin secretions have been identified to show antiviral activity. Our previously reported ESC-1GN, a peptide from the skin secretion of Hylarana guentheri, displayed good antimicrobial and antiinflammatory effects. Here, we found that ESC-1GN possessed significant antiviral effects against IAVs. Moreover, ESC-1GN could inhibit the entry of divergent H5N1 and H1N1 virus strains with the IC50 values from 1.29 to 4.59(mu)M. Mechanism studies demonstrated that ESC-1GN disrupted membrane fusion activity of IAVs by interaction with HA2 subunit. The results of site-directed mutant assay and molecular docking revealed that E105, N50 and the residues around them on HA2 subunit could form hydrogen bonds with amino acid on ESC-1GN, which were critical for ESC-1GN binding to HA2 and inhibiting the entry of IAVs. Altogether, these not only suggest that ESC-1GN maybe represent a new type of excellent template designing drugs against IAVs, but also it may shed light on the immune mechanism and survival strategy of H.guentheri against viral pathogens. [GRAPHICS] .
引用
收藏
页码：757 / 765
页数：9
相关论文
共 45 条
[1]   Laridae: A neglected reservoir that could play a major role in avian influenza virus epidemiological dynamics [J].
Arnal, Audrey ;
Vittecoq, Marion ;
Pearce-Duvet, Jessica ;
Gauthier-Clerc, Michel ;
Boulinier, Thierry ;
Jourdain, Elsa .
CRITICAL REVIEWS IN MICROBIOLOGY, 2015, 41 (04) :508-519
[2]   In vitro antiviral activity of dermaseptins against herpes simplex virus type-1 [J].
Belaid, A ;
Aouni, M ;
Khelifa, R ;
Trabelsi, A ;
Jemmali, M ;
Hani, K .
JOURNAL OF MEDICAL VIROLOGY, 2002, 66 (02) :229-234
[3]   Influenza virus-related critical illness: prevention, diagnosis, treatment [J].
Chow, Eric J. ;
Doyle, Joshua D. ;
Uyeki, Timothy M. .
CRITICAL CARE, 2019, 23 (1)
[4]   Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus [J].
Claas, ECJ ;
Osterhaus, ADME ;
van Beek, R ;
De Jong, JC ;
Rimmelzwaan, GF ;
Senne, DA ;
Krauss, S ;
Shortridge, KF ;
Webster, RG .
LANCET, 1998, 351 (9101) :472-477
[5]   Brief report - Oseltamivir resistance during treatment of influenza A (H5N1) infection [J].
de Jong, MD ;
Thanh, TT ;
Khanh, TH ;
Hien, VM ;
Smith, GJD ;
Chau, NV ;
Cam, BV ;
Qui, PT ;
Ha, DQ ;
Guan, Y ;
Peiris, JSM ;
Hien, TT ;
Farrar, J .
NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (25) :2667-2672
[6]   D-Amino acids incorporation in the frog skin-derived peptide esculentin-1a(1-21)NH2 is beneficial for its multiple functions [J].
Di Grazia, Antonio ;
Cappiello, Floriana ;
Cohen, Hadar ;
Casciaro, Bruno ;
Luca, Vincenzo ;
Pini, Alessandro ;
Di, Y. Peter ;
Shai, Yechiel ;
Mangoni, Maria Luisa .
AMINO ACIDS, 2015, 47 (12) :2505-2519
[7]  
Fiore Anthony E., 2011, Morbidity and Mortality Weekly Report, V60, P1
[8]   Oligonol a low molecular weight polyphenol of lychee fruit extract inhibits proliferation of influenza virus by blocking reactive oxygen species-dependent ERK phosphorylation [J].
Gangehei, Leila ;
Ali, Muzammil ;
Zhang, Wei ;
Chen, Zhenping ;
Wakame, Koji ;
Haidari, Mehran .
PHYTOMEDICINE, 2010, 17 (13) :1047-1056
[9]   An Amphibian Host Defense Peptide Is Virucidal for Human H1 Hemagglutinin-Bearing Influenza Viruses [J].
Holthausen, David J. ;
Lee, Song Hee ;
Kumar, Vineeth T. V. ;
Bouvier, Nicole M. ;
Krammer, Florian ;
Ellebedy, Ali H. ;
Wrammert, Jens ;
Lowen, Anice C. ;
George, Sanil ;
Pillai, Madhavan Radhakrishna ;
Jacob, Joshy .
IMMUNITY, 2017, 46 (04) :587-595
[10]   Early steps of the conformational change of influenza virus hemagglutinin to a fusion active state -: Stability and energetics of the hemagglutinin [J].
Huang, Q ;
Sivaramakrishna, RP ;
Ludwig, K ;
Korte, T ;
Böttcher, C ;
Herrmann, A .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2003, 1614 (01) :3-13
12345