Three-photon imaging of mouse brain structure and function through the intact skull

被引：257

作者：

Wang, Tianyu ^{[1
]}

Ouzounov, Dimitre G. ^{[1
]}

Wu, Chunyan ^{[1
]}

Horton, Nicholas G. ^{[1
]}

Zhang, Bin ^{[2
]}

Wu, Cheng-Hsun ^{[2
]}

Zhang, Yanping ^{[2
,3
]}

Schnitzer, Mark J. ^{[2
,3
]}

Xu, Chris ^{[1
]}

机构：

[1] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14853 USA

[2] Stanford Univ, CNC Program, Stanford, CA 94305 USA

[3] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2018年 / 15卷 / 10期

基金：

美国国家科学基金会;

关键词：

CRANIAL WINDOW; THINNED-SKULL; LONG-TERM; MICROSCOPY; RESOLUTION; CORTEX; DEEP; MICE;

D O I：

10.1038/s41592-018-0115-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even when we used the same excitation wavelength and imaging system. Here we demonstrate three-photon imaging of vasculature through the adult mouse skull at >500-mu m depth, as well as GCaMP6s calcium imaging over weeks in cortical layers 2/3 and 4 in awake mice, with 8.5 frames per second and a field of view spanning hundreds of micrometers.

引用

页码：789 / +

页数：6

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