Regional distribution of urocortin-like immunoreactivity and expression of urocortin mRNA in the human brain

被引：67

作者：

Takahashi, K

Totsune, K

Sone, M

Murakami, O

Satoh, F

Arihara, Z

Sasano, H

Iino, K

Mouri, T

机构：

[1] Tohoku Univ, Sch Med, Dept Mol Biol, Aoba Ku, Sendai, Miyagi 98077, Japan

[2] Tohoku Univ, Sch Med, Dept Internal Med 2, Sendai, Miyagi 98077, Japan

[3] Tohoku Univ, Sch Med, Dept Pathol, Sendai, Miyagi 98077, Japan

[4] Natl Iwate Hosp, Dept Internal Med, Ichinoseki, Iwate 021, Japan

[5] Mouri Clin, Div Neuroendocrinol, Natori, Miyagi 98112, Japan

来源：

PEPTIDES | 1998年 / 19卷 / 04期

关键词：

urocortin; human brain; radioimmunoassay; RT-PCR; PCR;

D O I：

10.1016/S0196-9781(98)00012-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Regional distribution of urocortin-like immunoreactivity (UCN-LI) in the human brain was studied by radioimmunoassay and was compared with that of corticotropin-releasing hormone (CRH). In addition, the expression of UCN mRNA was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method. UCN-LI was detected in every region of brain examined, including hypothalamus, pens, cerebral cortex, and cerebellum. The concentrations of UCN-LI in the human brain were approximately 3 pmol/g wet weight in any brain region, and no marked regional difference was noted. On the other hand, the highest concentrations of CRH-LI were found in the frontal cortex, temporal cortex, and hypothalamus and the lowest in the pens. Reverse phase high-performance liquid chromatography of the UCN-LI in the human brain extract showed two immunoreactive peaks; one peak eluting earlier and one in the position of synthetic human UCN. RT-PCR showed that UCN mRNA was expressed in every region of brain examined. These findings indicated that UCN and UCN mRNA were widely expressed in the human brain. (C) 1998 Elsevier Science Inc.

引用

页码：643 / 647

页数：5

共 23 条

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