SEQUENCING PROTOCOLS TO GENOTYPE mdx, mdx4cv, AND mdx5cv MICE

被引：17

作者：

Banks, Glen B. ^{[1
]}

Combs, Ariana C. ^{[1
]}

Chamberlain, Jeffrey S. ^{[1
,2
,3
]}

机构：

[1] Univ Washington, Dept Neurol, Seattle, WA 98195 USA

[2] Univ Washington, Dept Med, Seattle, WA 98105 USA

[3] Univ Washington, Dept Biochem, Seattle, WA 98195 USA

来源：

MUSCLE & NERVE | 2010年 / 42卷 / 02期

关键词：

genotyping; mouse dystrophin gene; mdx mice; LINKED MUSCULAR-DYSTROPHY; MOUSE; MUTATIONS;

D O I：

10.1002/mus.21700

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx, mdx(4cv), and mdx(5cv) mice. This method clearly distinguishes between wildtype, heterozygous, and mutant transcripts, and thereby time and money can be saved by avoiding false positives. Muscle Nerve 42: 268-270, 2010

引用

页码：268 / 270

页数：3

共 7 条

[1] X-CHROMOSOME-LINKED MUSCULAR-DYSTROPHY (MDX) IN THE MOUSE [J].