Immunogenic and efficacious SARS-CoV-2 vaccine based on resistin-trimerized spike antigen SmT1 and SLA archaeosome adjuvant

被引：31

作者：

Akache, Bassel ^{[1
]}

Renner, Tyler M. ^{[1
]}

Tran, Anh ^{[1
]}

Deschatelets, Lise ^{[1
]}

Dudani, Renu ^{[1
]}

Harrison, Blair A. ^{[1
]}

Duque, Diana ^{[1
]}

Haukenfrers, Julie ^{[1
]}

Rossotti, Martin A. ^{[1
]}

Gaudreault, Francis ^{[1
]}

Hemraz, Usha D. ^{[2
]}

Lam, Edmond ^{[2
]}

Regnier, Sophie ^{[2
]}

Chen, Wangxue ^{[1
]}

Gervais, Christian ^{[1
]}

Stuible, Matthew ^{[1
]}

Krishnan, Lakshmi ^{[1
]}

Durocher, Yves ^{[1
]}

McCluskie, Michael J. ^{[1
]}

机构：

[1] Natl Res Council Canada, Human Hlth Therapeut, 1200 Montreal Rd, Ottawa, ON K1A 0R6, Canada

[2] Natl Res Council Canada, Aquat & Crop Resource Dev, 6100 Ave Royalmount, Montreal, PQ H4P 2R2, Canada

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

PROTEIN; NEUTRALIZATION; CHALLENGES; COVID-19; VECTORS; COV;

D O I：

10.1038/s41598-021-01363-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The huge worldwide demand for vaccines targeting SARS-CoV-2 has necessitated the continued development of novel improved formulations capable of reducing the burden of the COVID-19 pandemic. Herein, we evaluated novel protein subunit vaccine formulations containing a resistin-trimerized spike antigen, SmT1. When combined with sulfated lactosyl archaeol (SLA) archaeosome adjuvant, formulations induced robust antigen-specific humoral and cellular immune responses in mice. Antibodies had strong neutralizing activity, preventing viral spike binding and viral infection. In addition, the formulations were highly efficacious in a hamster challenge model reducing viral load and body weight loss even after a single vaccination. The antigen-specific antibodies generated by our vaccine formulations had stronger neutralizing activity than human convalescent plasma, neutralizing the spike proteins of the B.1.1.7 and B.1.351 variants of concern. As such, our SmT1 antigen along with SLA archaeosome adjuvant comprise a promising platform for the development of efficacious protein subunit vaccine formulations for SARS-CoV-2.

引用

页数：17

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