Engineered alkane-hydroxylating cytochrome P450BM3 exhibiting nativelike catalytic properties

被引：177
作者：
Fasan, Rudi [1 ]
Chen, Mike M. [1 ]
Crook, Nathan C. [1 ]
Arnold, Frances H. [1 ]
机构：
[1] CALTECH, Dept Chem & Chem Engn, Pasadena, CA 91125 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 44期
关键词：
alkane oxidation; biotransformations; cytochrome P450; directed evolution; protein engineering;
D O I：
10.1002/anie.200702616
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Divide, evolve, and conquer: A domain-based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole-cell bioconversions of propane to propanol under mild conditions, using air as oxidant. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：8414 / 8418
页数：5
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