Engineered alkane-hydroxylating cytochrome P450BM3 exhibiting nativelike catalytic properties

被引：179

作者：

Fasan, Rudi ^{[1
]}

Chen, Mike M. ^{[1
]}

Crook, Nathan C. ^{[1
]}

Arnold, Frances H. ^{[1
]}

机构：

[1] CALTECH, Dept Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 44期

关键词：

alkane oxidation; biotransformations; cytochrome P450; directed evolution; protein engineering;

D O I：

10.1002/anie.200702616

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Divide, evolve, and conquer: A domain-based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole-cell bioconversions of propane to propanol under mild conditions, using air as oxidant. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8414 / 8418

页数：5

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