Methylene Blue-Conjugated Hydrogel Nanoparticles and Tumor-Cell Targeted Photodynamic Therapy

被引：93

作者：

Hah, Hoe Jin ^{[1
]}

Kim, Gwangseong ^{[1
]}

Lee, Yong-Eun Koo ^{[1
]}

Orringer, Daniel A. ^{[2
]}

Sagher, Oren ^{[2
]}

Philbert, Martin A. ^{[3
]}

Kopelman, Raoul ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[2] Dept Neurosurg, Ann Arbor, MI 48109 USA

[3] Dept Environm Hlth Sci, Ann Arbor, MI 48109 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 01期

基金：

美国国家卫生研究院;

关键词：

hydrogels; methylene blue; nanoparticles; photodynamic therapy; tumor targeting; SINGLET OXYGEN; ENDOTHELIAL-CELLS; PHOTOSENSITIZERS; PRINCIPLES; ENCAPSULATION; INACTIVATION; DELIVERY; VIRUSES; SERIES;

D O I：

10.1002/mabi.201000231

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methylene blue-conjugated polyacrylamide nanoparticles are prepared through a microemulsion polymerization, after conjugation of the dye with a monomer. The nanoparticles have a 50-60 nm diameter in solution. This conjugation method enables a large increase in loading of methylene blue per nanoparticle and also minimizes dye leaching out of the nanoparticle. Furthermore, the dye content can be controlled by variation of the dye amount, enabling a more refined control of the singlet oxygen production ability. The nanoparticles are coated with F3 peptides, which give specific targeting to selected tumor cells, 9L, MDA-MB-435, and F98, in vitro. In addition, MTT assays reveal that the nanoparticles have no dark toxicity but excellent PDT efficacy increasing with the nanoparticle dose and irradiation time.

引用

页码：90 / 99

页数：10

共 40 条

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